Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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A novel benzimidazole derivative, MBIC inhibits tumor growth and promotes apoptosis via activation of ROS-dependent JNK signaling pathway in hepatocellular carcinoma.
Xiaoyun Dai,Lingzhi Wang,Amudha Deivasigamni,Chung Yeng Looi,Chandrabose Karthikeyan,Piyush Trivedi,Arunachalam Chinnathambi,Sulaiman Ali Alharbi,Frank Arfuso,Arunasalam Dharmarajan,Boon Cher Goh,Kam M. Hui,Alan Prem Kumar,Mohd Rais Mustafa,Gautam Sethi +14 more
TL;DR: It is found that MBIC inhibited cell viability in different hepatocellular carcinoma (HCC) cell lines without exerting significant cytotoxic effects on normal liver cells, and furthermore, MBIC significantly inhibited tumor growth in an orthotopic HCC mouse model.
Journal ArticleDOI
ROS in Platelet Biology: Functional Aspects and Methodological Insights
Elena Masselli,Giulia Pozzi,Mauro Vaccarezza,Prisco Mirandola,Daniela Galli,Marco Vitale,Cecilia Carubbi,Giuliana Gobbi +7 more
TL;DR: This review sought to elucidate molecular mechanisms underlying ROS production upon platelet activation and the effects of an altered redox balance on platelet function, focusing on the main advances that have been made in platelet redox biology.
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A dual-targeting, apoptosis-inducing organometallic half-sandwich iridium anticancer complex
Vojtech Novohradsky,Vojtech Novohradsky,Lenka Zerzankova,Jana Stepankova,Anna Kisova,Hana Kostrhunova,Zhe Liu,Peter J. Sadler,Jana Kasparkova,Viktor Brabec +9 more
TL;DR: Flow cytometric studies and impedance-based monitoring of cellular responses to 1 demonstrated that 1 acts more quickly than cisplatin to induce cell death and that 1 is a more effective apoptosis inducer than cis platin in particular in early stages of treatment, when the apoptotic effects predominate over necrosis.
Journal ArticleDOI
Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy
TL;DR: In this article, the use of two fluorescence probes: singlet oxygen sensor green (SOSG) detects 1O2; and 4-hydroxyphenyl-fluorescein (HPF) detects HO.
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The transcription factor Nrf2 promotes survival by enhancing the expression of uncoupling protein 3 under conditions of oxidative stress.
Andrea Anedda,Elia López-Bernardo,Bárbara Acosta-Iborra,M.Saadeh Suleiman,Manuel O. Landázuri,Susana Cadenas +5 more
TL;DR: Findings suggest that UCP3 functions as a member of the cellular antioxidant defense system that protects against oxidative stress in vivo, and identifies a novel regulatory process induced by an oxidative insult whereby the expression of the mitochondrial protein U CP3 is driven by the Nrf2 transcription factor, which decreases ROS production and prevents cell death.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.