Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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Salinity Variation in a Mangrove Ecosystem: A Physiological Investigation to Assess Potential Consequences of Salinity Disturbances on Mangrove Crabs.
Dimitri Theuerkauff,Georgina A. Rivera-Ingraham,J.A.C. Roques,Laurence Azzopardi,Marine Bertini,Mathilde Lejeune,Emilie Farcy,Jehan-Hervé Lignot,Elliott Sucré +8 more
TL;DR: While environmental salinity alone cannot directly explain fiddler and red mangrove crab distributions, the data suggest that salinity selection is indeed influenced by specific physiological adjustments.
Journal ArticleDOI
Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila
TL;DR: It is found that inhibition of the transcription factor Nrf2 further enhances the toxicity caused by CuONPs and that oxidative stress serves as one of the primary causes of CuONP-induced toxicity in Drosophila.
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Mitochondrial Peroxiredoxin-3 protects against hyperglycemia induced myocardial damage in Diabetic cardiomyopathy
TL;DR: It is suggested that diabetes induced cardiomyopathy can be prevented by elevating Prx-3 levels thereby providing extensive protection to the diabetic heart.
Journal ArticleDOI
Intracellular Microreactors as Artificial Organelles to Conduct Multiple Enzymatic Reactions Simultaneously.
Maria Godoy-Gallardo,Cédric Labay,Michelle M. T. Jansman,Pramod Kumar Ek,Leticia Hosta-Rigau +4 more
TL;DR: This report on the first microreactor conducting multiple enzymatic reactions simultaneously inside a cell is a considerable step in the field of artificial organelles, which aims to substitute for missing cellular function by replenishing a specific cellular task.
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Mitochondrial ROS cause motor deficits induced by synaptic inactivity: Implications for synapse pruning
Eva Sidlauskaite,Jack W. Gibson,Ian L. Megson,Philip D. Whitfield,Artak Tovmasyan,Ines Batinic-Haberle,Michael P. Murphy,Peter R. Moult,James N. Cobley +8 more
TL;DR: This paper showed that synaptic inactivity increased mitochondrial reactive oxygen species (ROS) production, and chemically heterogeneous antioxidants rescue synapse inactivity induced motor deficits in developing Xenopus laevis tadpoles.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.