Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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γ-Glutamyltransferase enzyme activity of cancer cells modulates L-γ-glutamyl-p-nitroanilide (GPNA) cytotoxicity.
Alessandro Corti,Silvia Dominici,Simona Piaggi,E. Belcastro,E. Belcastro,E. Belcastro,Martina Chiu,Giuseppe Taurino,Simone Pacini,Ovidio Bussolati,Alfonso Pompella +10 more
TL;DR: The GGT-catalyzed hydrolysis of GPNA produced cytotoxic effects in lung cancer A549 cells, resulting from the release of metabolite p-nitroaniline (PNA) rather than from the inhibition of Gln uptake, indicating the mechanism of action of GP NA is more complex than believed.
Journal ArticleDOI
Phthalocyanine-based photosensitizer with tumor-pH-responsive properties for cancer theranostics
Shufeng Yan,Jincan Chen,Liangzhi Cai,Liangzhi Cai,Peng Xu,Yaxin Zhang,Shijie Li,Ping Hu,Xueyuan Chen,Mingdong Huang,Mingdong Huang,Zhuo Chen +11 more
TL;DR: A pH-responsive photosensitizer synthesized by conjugating zinc phthalocyanine with 2,4,6-tris(N,N-dimethylaminomethyl) phenoxy (TAP), which exhibited high phototoxicity at pH 6.5 but no obvious photot toxicity at physiological pH 7.4, may open up a new avenue for tumor-targeted and image-guided cancer theranostics in PDT.
Journal ArticleDOI
Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants
TL;DR: In this paper, the authors compared five ROS-related and two non-ROS-related reagents in the roots of Zea mays germinated and growing in 1 or 100µM cadmium-enriched solution.
Journal ArticleDOI
Redox biology in pulmonary arterial hypertension (2013 Grover Conference Series).
Joshua P. Fessel,James West +1 more
TL;DR: This review has attempted to summarize some of the important existing studies, to point out areas of overlap between oxidative stress and metabolic dysfunction, and to do so under the unifying heading of redox biology, to advance the discussion of carbon-substrate metabolism beyond a focus on glucose and its fate in the cell to encompass other carbon substrates.
Journal ArticleDOI
Myostatin knockout induces apoptosis in human cervical cancer cells via elevated reactive oxygen species generation.
Ying-Qian Han,Sheng-Li Ming,Hong-Tao Wu,Lei Zeng,Gen Ba,Jian Li,Weifei Lu,Jie Han,Qia-Jun Du,Miao-Miao Sun,Guo-Yu Yang,Jiang Wang,Bei-Bei Chu +12 more
TL;DR: Limiting the production and function of Mstn may be an effective therapeutic intervention during cancer progression and muscle loss in cachexia because of its role in regulating mitochondrial metabolism and apoptosis within cancer cells.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.