Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
More filters
Journal ArticleDOI
Clinical Relevance and Role of Neuronal AT 1 Receptors in ADAM17-Mediated ACE2 Shedding in Neurogenic Hypertension
Jiaxi Xu,Srinivas Sriramula,Huijing Xia,Lisa Moreno-Walton,Frank Culicchia,Oliver Domenig,Marko Poglitsch,Eric Lazartigues +7 more
TL;DR: It is demonstrated that angiotensin II type 1 receptors promote ADAM17-mediated ACE2 shedding in the brain of hypertensive patients, leading to a loss in compensatory activity during neurogenic hypertension.
Journal ArticleDOI
Oxidative Stress and Huntington's Disease: The Good, The Bad, and The Ugly.
Amit Kumar,Rajiv R. Ratan +1 more
TL;DR: Oxidative stress has long been held to be one of the key players in disease progression for Huntington’s disease (HD), and this review will critically review this evidence, drawing some intermediate conclusions, and provide a framework for thinking about the role of oxidative stress in the pathophysiology of HD.
Journal ArticleDOI
Heterogeneous Assembled Nanocomplexes for Ratiometric Detection of Highly Reactive Oxygen Species in Vitro and in Vivo
TL;DR: Binary heterogeneous nanocomplexes constructed from gold clusters and carbon dots, with high physiological stability and excellent biocompatibility, C-dots-AuNC allows for the detection of hROS in the model of local ear inflammation.
Journal ArticleDOI
Age-associated declines in mitochondrial biogenesis and protein quality control factors are minimized by exercise training
Erika Koltai,Nikolett Hart,Albert W. Taylor,Sataro Goto,Jenny K. Ngo,Kelvin J.A. Davies,Zsolt Radak +6 more
TL;DR: It is concluded that exercise training can help minimize detrimental skeletal muscle aging deficits by improving mitochondrial protein quality control and biogenesis.
Journal ArticleDOI
Altered Mitochondrial Function, Mitochondrial DNA and Reduced Metabolic Flexibility in Patients With Diabetic Nephropathy.
Anna Czajka,Saima Ajaz,Luigi Gnudi,Chandani Kiran Parsade,Peter M. Jones,Fiona Reid,Afshan N. Malik +6 more
TL;DR: Data show that MtDNA changes precede bioenergetic dysfunction and that patients with DN have impaired mitochondrial metabolism compared to DC, leading us to propose that systemic mitochondrial dysfunction initiated by glucose induced MtDNA damage may be involved in the development of DN.
References
More filters
Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
Journal ArticleDOI
Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.