Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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Fe-MIL-100 as drug delivery system for asthma and chronic obstructive pulmonary disease treatment and diagnosis
TL;DR: Results confirmed that Fe-MIL-100 can be used safely as carrier for potential application in inhalable treatments and stressed the measurement of stress induced in the living cells by Fe-mil-100 by a measurement of the level of oxygen reactive species generation.
Journal ArticleDOI
Silica particles cause NADPH oxidase-independent ROS generation and transient phagolysosomal leakage
TL;DR: Phagosomes containing silica particles leak their contents into the cytoplasm, leading to apoptosis, and leakage has been linked to ROS.
Journal ArticleDOI
Benzocoumarin Hydrazine: A Large Stokes Shift Fluorogenic Sensor for Detecting Carbonyls in Isolated Biomolecules and in Live Cells
Kamalika Mukherjee,Tak Ian Chio,Han Gu,Abhijit Banerjee,Anthony M. Sorrentino,Dan L. Sackett,Susan Bane +6 more
TL;DR: A new fluorescent sensor for biomolecule carbonyl detection: a hydrazine derivative of a benzocoumarin, 7-hydrazinyl-4-methyl-2H-benzo[h]chromen-2-one (BzCH), which meets the requirements of live cell detection and is especially well suited for live cell studies.
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ESR evidence for in vivo formation of free radicals in tissue of mice exposed to single-walled carbon nanotubes.
Anna A. Shvedova,Anna A. Shvedova,Elena R. Kisin,Ashley R. Murray,Ange Mouithys-Mickalad,Krisztian Stadler,Ronald P. Mason,Maria B. Kadiiska +7 more
TL;DR: Exposure to partially purified SWCNTs resulted in the augmentation of oxidative stress as evidenced by ESR detection of α-(4-pyridyl-1-oxide)-N-tert-butylnitrone spin-trapped carbon-centered lipid-derived radicals recorded shortly after the treatment.
Journal Article
Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe
TL;DR: A peroxiredoxin-2-based FRET probe that is sensitive enough to measure changes in H2O2 concentration in response to the cancer therapeutic piperlongumine, and reveals peroxide-induced oxidation in human cells below the detection limit of current probes.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.