Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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Sea Squirt (Halocynthia roretzi) Hydrolysates Induce Apoptosis in Human Colon Cancer HT-29 Cells through Activation of Reactive Oxygen Species.
TL;DR: Results suggest that SSQ may be useful for functional food ingredients and/or nutraceuticals as well as anticancer effect on human colon cancer HT-29 cells.
Journal ArticleDOI
Expression and functional analysis of the hydrogen peroxide biosensors HyPer and HyPer2 in C2C12 myoblasts/myotubes and single skeletal muscle fibres.
Escarlata Fernández-Puente,Manuel Sánchez-Martín,Jorge de Andrés,Lorena Rodríguez-Izquierdo,Lucía Méndez,Jesus Palomero +5 more
TL;DR: The results show that the expression of the HyPer biosensor in skeletal muscle cells is possible, and it is demonstrated that HyPer is functional and that this biosensor detects changes and fluctuations in intracellular H 2 O 2 in a reversible manner.
Journal Article
A novel approach to study oxidative stress in thyroid diseases: a preliminary study
TL;DR: This preliminary study demonstrates the opportunity to use the coupling of the EPR-spin trapping technique with the hydroxylamine 1-hydroxy-3-carboxy-pyrrolidine, to detect oxidative stress in the human blood of patients with thyroid disease, and confirms that this is a sensible, precise and valid method to study the oxidative stress.
Journal ArticleDOI
Reactive oxygen species are physiological mediators of the noradrenergic signaling pathway in the mouse supraoptic nucleus.
Ronald St-Louis,Ronald St-Louis,Ronald St-Louis,Caroline Parmentier,Caroline Parmentier,Caroline Parmentier,Valérie Grange-Messent,Valérie Grange-Messent,Valérie Grange-Messent,Sakina Mhaouty-Kodja,Sakina Mhaouty-Kodja,Sakina Mhaouty-Kodja,Hélène Hardin-Pouzet,Hélène Hardin-Pouzet,Hélène Hardin-Pouzet +14 more
TL;DR: Evidence is provided of the physiological importance of free radicals, which should no longer be considered solely as cytotoxic factors, and the mechanisms linking free radicals and noradrenaline (NA)-induced regulation of AVP.
Journal ArticleDOI
Detection of hydrogen peroxide production in the isolated rat lung using Amplex red.
Said H. Audi,Nina Friedly,Ranjan K. Dash,Andreas M. Beyer,Anne V. Clough,Elizabeth R. Jacobs +5 more
TL;DR: Results suggest complex II as a potentially important source and/or regulator of mitochondrial H2O2, and that most of acute hyperoxia-enhanced lung rate of H2 O2 release is from nonmitochondrial rather than mitochondrial sources.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.