Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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Flow cytometric HyPer-based assay for hydrogen peroxide.
Olga G. Lyublinskaya,S.A. Antonov,S.G. Gorokhovtsev,N. A. Pugovkina,Ju. S. Kornienko,Ju. S. Ivanova,Alla N. Shatrova,N. D. Aksenov,V. V. Zenin,N. N. Nikolsky +9 more
TL;DR: HyPer can be used in multi‐parameter flow cytometry studies as a highly sensitive indicator of intracellular H2O2 as well as in ratiometric imaging of H2 O2 fluxes in living cells.
Journal ArticleDOI
Synthesis and biological evaluation of naphthalimide-polyamine conjugates modified by alkylation as anticancer agents through p53 pathway.
Fujun Dai,Haoying He,Xiaojuan Xu,Shuai Chen,Chaojie Wang,Chenyang Feng,Zhiyong Tian,Huanyang Dong,Songqiang Xie +8 more
TL;DR: A series of novel naphthalimide-polyamine conjugates modified by alkylation at the terminal of the polyamine chain were synthesized and revealed that the length of thepolyamine chain and the terminal alkyl group had influences on anticancer activities.
Journal ArticleDOI
Horseradish Peroxidase-Encapsulated Hollow Silica Nanospheres for Intracellular Sensing of Reactive Oxygen Species.
Hsin Yi Chen,Si Han Wu,Chien Tsu Chen,Yi Ping Chen,Feng Peng Chang,Fan Ching Chien,Chung-Yuan Mou +6 more
TL;DR: A new approach for intracellular sensing of ROS by using horseradish peroxidase-encapsulated hollow silica nanospheres (designated HRP@HSNs), with satisfactory catalytic activity, cell membrane permeability, and biocompatibility, was prepared via a microemulsion method.
Journal ArticleDOI
Combining analytical and in vitro techniques for comprehensive assessments of chemical exposure and effect in green sea turtles (Chelonia mydas).
Kimberly A. Finlayson,Frederic D.L. Leusch,Cesar A. Villa,Colin J. Limpus,Jason P. van de Merwe +4 more
TL;DR: A combination of chemical analysis and effects-based bioassays were used to provide complementary information on chemical exposure and effects for three green turtle foraging populations (Chelonia mydas) in southern Queensland, Australia as discussed by the authors.
Journal ArticleDOI
Drp1-dependent mitochondrial fission mediates corneal injury induced by alkali burn
Kun Zhang,Miao-Yu Guo,Qiu-Gen Li,Xiao-Hua Wang,Yu-Ying Wan,Zhang-Jian Yang,Min He,Yun-Min Yi,Li-Ping Jiang,Xin-Hui Qu,Xiao-Jian Han +10 more
TL;DR: In this paper, the expression and phosphorylation (Ser616) of the mitochondrial fission protein Drp1 were upregulated at day 3 after alkali burn, while mitochondrial fusion protein Mfn2 was downregulated.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
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Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.