Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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Journal ArticleDOI
Pyrocatalytic oxidation – strong size-dependent poling effect on catalytic activity of pyroelectric BaTiO3 nano- and microparticles
Sascha Raufeisen,Peter Neumeister,Johannes R. Buchheim,Michael Stelter,Michael Stelter,Patrick Braeutigam +5 more
TL;DR: This study greatly improves the understanding about the role of important material properties and electric poling on pyrocatalytic activity, thus enabling an effective catalyst design and future strategy for maximizing the catalytic performance of pyroCatalysts was developed.
Journal ArticleDOI
Anti-inflammatory effect of water extracts obtained from doenjang in LPS-stimulated RAW 264.7 cells
TL;DR: Inhibition of inflammaproduction of inflammatory mediator is the important therapeutic target for healing of inflammation-related diseases (Ko & Jeon, 2015).
Journal ArticleDOI
Sch9 regulates intracellular protein ubiquitination by controlling stress responses
Beibei Qie,Zhou Lyu,Lei Lyu,Jun Liu,Xuejie Gao,Yanyan Liu,Wei Duan,Nian-Hui Zhang,Linfang Du,Ke Liu +9 more
TL;DR: It is demonstrated here that the decrease of ubiquitinated proteins in sch9Δ cells in log phase is not caused by changes in ubiquitin expression, proteasome activity, or autophagy, but by enhanced expression of stress response factors and a decreased level of oxidative stress.
Book ChapterDOI
Redox Homeostasis in Age-Related Muscle Atrophy.
TL;DR: This chapter summarizes the current evidence that has associated disrupted redox homeostasis and muscle atrophy as a result of skeletal muscle inactivity and aging.
Journal ArticleDOI
Effects of new tetrahydroquinoline-isoxazole hybrids on bioenergetics of hepatocarcinoma Hep-G2 cells and rat liver mitochondria.
Marilyn Rocío Álvarez Santos,Yenny Bueno Duarte,Fausto M. Güiza,Arnold R. Romero Bohórquez,Stelia Carolina Mendez-Sanchez +4 more
TL;DR: The results show that THQ-ISX hybrids are promising compounds for hepatoma cancer treatment and that the phenyl substituent (Y= H) in the ISX scaffold intensifies both, the cytotoxicity in Hep-G2 cells and, inhibition of electron transport through complex I of the mitochondrial respiratory chain.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
Journal ArticleDOI
Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.