Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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The use of a chemically defined artificial diet as a tool to study Aedes aegypti physiology.
Octavio A. C. Talyuli,Octavio A. C. Talyuli,Vanessa Bottino-Rojas,Mabel L. Taracena,Ana Luiza Ferreira Macedo Soares,Ana Luiza Ferreira Macedo Soares,Jose Henrique M. Oliveira,Pedro L. Oliveira +7 more
TL;DR: Depending on the nutritional life history of the mosquito since the larval stage, the presence of cholesterol in the diet increased egg development, suggesting that the teneral reserves in the newly hatched female are determinant of reproductive performance.
Journal ArticleDOI
Recent developments of fluorescent probes for detection and bioimaging of nitric oxide.
TL;DR: This review focuses on organic small-molecule fluorescent probes for selective detection and imaging of endogenous NO in vitro and in vivo and suggests that fluorescence imaging is a vital and rapidly technology for real-time detection of NO in biological system.
Journal ArticleDOI
Biological chemistry of superoxide radicals
TL;DR: The properties and biological chemistry of superoxide radicals are considered, how they are produced, what biomolecules they react with, and the reactions that contribute to their toxicity.
Journal ArticleDOI
Rutin protects against H2O2-triggered impaired relaxation of placental arterioles and induces Nrf2-mediated adaptation in Human Umbilical Vein Endothelial Cells exposed to oxidative stress
Mireille M.J.P.E. Sthijns,Mireille M.J.P.E. Sthijns,Paul M. H. Schiffers,Ger M.J. Janssen,Kristien J.A. Lemmens,Bart Ides,Philippe Vangrieken,Freek G. Bouwman,Edwin C. M. Mariman,Irina Pader,Elias S.J. Arnér,Katarina Johansson,Aalt Bast,Guido R.M.M. Haenen +13 more
TL;DR: Rutin functions as an antioxidant and is oxidized into a quinone that upregulates the Nrf2-mediated endogenous antioxidant response, which suggests that rutin selectively exerts its protective effects in regions with increased oxidative stress, and explains how r Rutin reduces the risk of developing CVD.
Journal ArticleDOI
Fluorescent in vivo imaging of reactive oxygen species and redox potential in plants.
Cristina Ortega-Villasante,Stefan Burén,Alfonso Blázquez-Castro,Ángel Barón-Sola,Luis E. Hernández +4 more
TL;DR: This review summarized the present knowledge for in vivo plant ROS imaging and detection, using both chemical probes and fluorescent protein‐based biosensors, to fully acknowledge the remarkably complex plant ROS signalling networks.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
Journal ArticleDOI
Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.