Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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Protective effect of selegiline on cigarette smoke-induced oxidative stress and inflammation in rat lungs in vivo
TL;DR: Inhibition of MAO-B may provide a promising therapeutic strategy for CS-mediated oxidative stress and inflammation in acute CS-exposed rat lungs.
Journal ArticleDOI
Iron and copper complexes with antioxidant activity as inhibitors of the metastatic potential of glioma cells
Joana F. Guerreiro,Joana F. Guerreiro,Marco Antônio G. B. Gomes,Francesca Pagliari,Jeannette Jansen,Jeannette Jansen,Maria Grazia Marafioti,Clelia Nisticò,Rachel Hanley,Rachel Hanley,Rafael O. Costa,Sarah S. Ferreira,Filipa Mendes,Christiane Fernandes,Adolfo Horn,Luca Tirinato,Luca Tirinato,Joao Seco,Joao Seco +18 more
TL;DR: The results obtained describe the novel use of iron and copper complexes as agents that are able to interfere with regulation of EMT and the invasive behavior of glioma cells, an application that deserves to be further explored.
Journal ArticleDOI
H2O2/HOCl-based fluorescent probes for dynamically monitoring pathophysiological processes
TL;DR: The optical properties of H2O2/HOCl-responsive fluorescent probes are reviewed and effective distribution and dynamic monitoring are focused on by using pathophysiological models.
Journal ArticleDOI
TiCaPCON-Supported Pt- and Fe-Based Nanoparticles and Related Antibacterial Activity.
Viktor A. Ponomarev,A. N. Sheveyko,Elizaveta S. Permyakova,Jihyung Lee,Andrey A. Voevodin,Diana Berman,Anton Manakhov,Miroslav Michlíček,Miroslav Michlíček,Pavel V. Slukin,Viktoriya V Firstova,Sergey G. Ignatov,I. V. Chepkasov,Zakhar I. Popov,Dmitry V. Shtansky +14 more
TL;DR: By eliminating the potential contribution of ions and ROS, it is shown that bacteria can be killed using direct microgalvanic interactions and provides new insights in understanding and designing of antibacterial yet biologically safe surfaces.
Journal ArticleDOI
Commercial single-walled carbon nanotubes effects in fibrinolysis of human umbilical vein endothelial cells.
Yury Rodríguez-Yáñez,Daniel Bahena-Uribe,Bibiana Chávez-Munguía,Rebeca López-Marure,Stuart González-Monroy,Bulmaro Cisneros,Arnulfo Albores +6 more
TL;DR: The generation of endothelial dysfunction, as shown by the altered expression of genes and proteins involved in fibrinolysis, suggest that SWCNTs display pro-thrombotic effects.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
Journal ArticleDOI
Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.