Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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Myeloperoxidase induces the priming of platelets
Hana Kolarova,Anna Klinke,Silvie Kremserová,M. Adam,Michaela Pekarova,Stephan Baldus,Jason P. Eiserich,Lukáš Kubala +7 more
TL;DR: It can be suggested that MPO can interact with and activate platelets, which can induce priming of platelets and contribute to the development of chronic inflammatory processes in vessels.
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Systematic in vitro nanotoxicity study on anodic alumina nanotubes with engineered aspect ratio: Understanding nanotoxicity by a nanomaterial model
Ye Wang,Gagandeep Kaur,Aneta Zysk,Vasilios Liapis,Shelley Hay,Abel Santos,Dusan Losic,Andreas Evdokiou +7 more
TL;DR: The toxic AR window of AANTs was determined to be 7.8, which is shorter than that of other previously reported HAR nanomaterials, which provides a promising opportunity to control the nanotoxicity of HAR nanmaterials for their advanced drug delivery application.
Journal ArticleDOI
Aging related functional and structural changes in the heart and aorta: MitoTEMPO improves aged-cardiovascular performance.
Yusuf Olgar,Sinan Degirmenci,Aysegul Durak,Deniz Billur,Belgin Can,Gizem Kayki-Mutlu,Ebru Arioglu-Inan E,Belma Turan +7 more
TL;DR: The present data strongly support the important role of mitochondrial oxidative stress in the development of aged‐related insufficiencies and that antioxidant strategies specifically targeting this organelle could have therapeutic benefit in aging‐associated complications.
Journal ArticleDOI
Neutrophil-generated HOCl leads to non-specific thiol oxidation in phagocytized bacteria.
Adriana Degrossoli,Alexandra Müller,Kaibo Xie,Jannis F. Schneider,Verian Bader,Konstanze F. Winklhofer,Andreas J. Meyer,Lars I. Leichert +7 more
TL;DR: It is shown that bacteria exposed to a neutrophil-like cell line experience a rapid and massive oxidation of cytosolic thiols and this massive breakdown of the thiol redox homeostasis was dependent on phagocytosis, presence of NADPH oxidase and ultimately myeloperoxidase.
Journal ArticleDOI
Nuclear Factor Erythroid 2-Related Factor 2 in Regulating Cancer Metabolism.
TL;DR: The molecular characteristics of theNRF2 pathway are reviewed, its interactions with the cancer hallmarks previously listed are discussed, and large-scale genomic and transcriptomic studies are missing to correlate the clinical outcome with the activity of the NRF2 system.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.