Spatiotemporal analysis of human intestinal development at single-cell resolution
David Fawkner-Corbett,Agne Antanaviciute,Kaushal Parikh,Marta Jagielowicz,Marta Jagielowicz,A S Gerós,A S Gerós,Tarun Gupta,Tarun Gupta,Neil Ashley,Doran Khamis,Darren J. Fowler,Edward Morrissey,C Cunningham,Johnson Prv.,Hashem Koohy,Hashem Koohy,Alison Simmons,Alison Simmons +18 more
TLDR
In this paper, single-cell RNA sequencing and spatial transcriptomics were used to characterize intestinal morphogenesis through time and identify 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones.About:
This article is published in Cell.The article was published on 2021-02-04 and is currently open access. It has received 189 citations till now. The article focuses on the topics: Population & Morphogen.read more
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Exploring tissue architecture using spatial transcriptomics.
TL;DR: Spatial transcriptomics can also be used for hypothesis testing using experimental designs that compare time points or conditions, including genetic or environmental perturbations as mentioned in this paper, and is naturally amenable to integration with other data modalities, providing an expandable framework for insight into tissue organization.
Journal ArticleDOI
Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics
TL;DR: In this paper, a suite of recently developed techniques that localize RNA within tissue, including multiplexed in situ hybridization and in situ sequencing (here defined as high-plex RNA imaging) and spatial barcoding, can help address this issue.
Journal ArticleDOI
Cell2location maps fine-grained cell types in spatial transcriptomics
Vitalii Kleshchevnikov,Artem Shmatko,Emma Dann,Alexander Aivazidis,Hamish W King,Tong Li,Rasa Elmentaite,Artem Lomakin,Veronika R. Kedlian,Adam Gayoso,Mika Sarkin Jain,Jun Sung Park,Lauma Ramona,Elizabeth Tuck,Anna Arutyunyan,Roser Vento-Tormo,Moritz Gerstung,Louisa K. James,Oliver Stegle,Omer Ali Bayraktar +19 more
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Cells of the human intestinal tract mapped across space and time.
Rasa Elmentaite,Natsuhiko Kumasaka,Kenny Roberts,Aaron M. Fleming,Emma Dann,Hamish W King,Vitalii Kleshchevnikov,Monika Dabrowska,Sophie Pritchard,Liam Bolt,Sara F. Vieira,Lira Mamanova,Ni Huang,Francesca Perrone,Issac Goh Kai’En,Steven Lisgo,Matilda Katan,Steven Leonard,Thomas R. W. Oliver,Thomas R. W. Oliver,C. Elizabeth Hook,Komal Nayak,Lia S. Campos,Cecilia Domínguez Conde,Emily Stephenson,Justin Engelbert,Rachel A. Botting,Krzysztof Polanski,Stijn van Dongen,Minal Patel,Michael D Morgan,Michael D Morgan,John C. Marioni,John C. Marioni,John C. Marioni,Omer Ali Bayraktar,Kerstin B Meyer,Xiaoling He,Roger A. Barker,Holm H. Uhlig,Holm H. Uhlig,Krishnaa T. Mahbubani,Kourosh Saeb-Parsy,Matthias Zilbauer,Menna R. Clatworthy,Menna R. Clatworthy,Muzlifah Haniffa,Muzlifah Haniffa,Kylie R. James,Kylie R. James,Sarah A. Teichmann,Sarah A. Teichmann +51 more
TL;DR: The cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures as discussed by the authors, using single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions of the developing and up to 11 distinct anatomical regions in the healthy human gut.
Journal ArticleDOI
Regulation of Neurotransmitters by the Gut Microbiota and Effects on Cognition in Neurological Disorders.
TL;DR: In this article, the authors summarize the mechanisms whereby the gut microbiota regulate the production, transportation, and functioning of neurotransmitters, and discuss how microbiome dysbiosis affects cognitive function, especially in neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
References
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