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Supplementary Materials for Host-Derived Nitrate Boosts Growth of E. coli in the Inflamed Gut
Sebastian E. Winter,Maria G. Winter,Mariana N. Xavier,A. Marijke Keestra,Richard Laughlin,Gabriel Gomez,Jing Wu,Ina Popova,Sanjai J. Parikh,L. Garry Adams,Renée M. Tsolis,Andreas J. Bäumler +11 more
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The article was published on 2013-01-01 and is currently open access. It has received 505 citations till now.read more
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Metagenomic Alterations in Gut Microbiota Precede and Predict Onset of Colitis in the IL10 Gene-Deficient Murine Model.
Jun Miyoshi,Sonny T. M. Lee,Megan Kennedy,Mora Puertolas,Mary Frith,Jason C. Koval,Sawako Miyoshi,Dionysios A. Antonopoulos,Vanessa Leone,Eugene B. Chang +9 more
TL;DR: Support is provided that functional metagenomic profiling of gut microbes has potential and promise meriting further study for development of tools to assess risk and manage human IBD.
Posted ContentDOI
Horizontal gene transfer in the human and skin commensal Malassezia: a bacterially-derived flavohemoglobin is required for NO resistance and host interaction
Giuseppe Ianiri,Marco A. Coelho,Fiorella Ruchti,Florian Sparber,Timothy J. McMahon,Ci Fu,Madison J. Bolejack,Olivia Donovan,Hayden Smutney,Peter J. Myler,Peter J. Myler,Fred S. Dietrich,David A. Fox,Salomé LeibundGut-Landmann,Joseph Heitman +14 more
TL;DR: It is demonstrated that both bacterially-derived flavohemoglobins are cytoplasmic proteins required for nitric oxide detoxification and nitrosative stress resistance under aerobic conditions, and in vivo pathogenesis is independent of Malassezia flavOhemoglobin.
Posted ContentDOI
Establishing the phenotypic basis of adherent-invasive Escherichia coli (AIEC) pathogenicity in intestinal inflammation
Hatem Kittana,João Carlos Gomes-Neto,Kari Heck,Jason Sughroue,Yibo Xian,Sara Mantz,Rafael R. Segura Munoz,Liz A. Cody,Robert Schmaltz,Christopher L. Anderson,Rodney A. Moxley,Jesse M. Hostetter,Samodha C. Fernando,Jennifer Clarke,Stephen D. Kachman,Clayton E. Cressler,Andrew K. Benson,Jens Walter,Amanda E. Ramer-Tait +18 more
TL;DR: The findings do not support the existence of an AIEC pathovar that can be clearly separated from commensal E. coli, but intracellular survival/replication phenotypes do contribute to murine intestinal inflammation, suggesting that the AIEC overgrowth observed in human IBD makes a causal contribution to disease.
Negative Regulators of Colonic Peripheral Regulatory T Cell Development
TL;DR: Data show that TCR specificity can determine regulatory vs effector T cell fates in the same host, and imply the existence of multiple microenvironments that direct distinct T cell differentiation pathways during intestinal inflammation.
Journal ArticleDOI
Predicting Inflammatory Bowel Disease Symptoms Onset: Nitrous Take on Gut Bacteria Is No Laughing Matter.
TL;DR: An important proof-of-concept study is presented that provides a new perspective on mining deep functional metagenomic data to predict inflammatory bowel disease (IBD) susceptibility and should lead to complement purely annotation and database-driven biomarker discovery with annotationagnostic workflows that identify new meetagenomic content that is predictive of disease susceptibility and treatment outcomes.
References
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
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A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
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Paul B. Eckburg,Elisabeth M. Bik,Charles N. Bernstein,Elizabeth Purdom,Les Dethlefsen,Michael Sargent,Steven R. Gill,Karen E. Nelson,David A. Relman,David A. Relman,David A. Relman +10 more
TL;DR: A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms, and significant intersubject variability and differences between stool and mucosa community composition were discovered.
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Obesity alters gut microbial ecology
Ruth E. Ley,Fredrik Bäckhed,Peter J. Turnbaugh,Catherine A. Lozupone,Robin D. Knight,Jeffrey I. Gordon +5 more
TL;DR: Analysis of the microbiota of genetically obese ob/ob mice, lean ob/+ and wild-type siblings, and their ob/+ mothers, all fed the same polysaccharide-rich diet, indicates that obesity affects the diversity of the gut microbiota and suggests that intentional manipulation of community structure may be useful for regulating energy balance in obese individuals.
Journal ArticleDOI
Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases
Daniel N. Frank,Allison L. St. Amand,Robert A. Feldman,Edgar C. Boedeker,Noam Harpaz,Norman R. Pace +5 more
TL;DR: Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances.