Open Access
Supplementary Materials for Host-Derived Nitrate Boosts Growth of E. coli in the Inflamed Gut
Sebastian E. Winter,Maria G. Winter,Mariana N. Xavier,A. Marijke Keestra,Richard Laughlin,Gabriel Gomez,Jing Wu,Ina Popova,Sanjai J. Parikh,L. Garry Adams,Renée M. Tsolis,Andreas J. Bäumler +11 more
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The article was published on 2013-01-01 and is currently open access. It has received 505 citations till now.read more
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Identification of simplified microbial communities that inhibit Clostridioides difficile infection through dilution/extinction
Jennifer M. Auchtung,Jennifer M. Auchtung,Eva C. Preisner,James J. Collins,James J. Collins,Armando I. Lerma,Armando I. Lerma,Robert A. Britton +7 more
TL;DR: An experimental platform to simplify complex communities of fecal bacteria through dilution and rapidly screen for their ability to inhibit C. difficile in vitro is developed and validated and identifies four new simplified communities of microbes with potential for development of new therapies to treat C.difficile disease in humans.
Journal ArticleDOI
Metabolic Modeling of Clostridium difficile Associated Dysbiosis of the Gut Microbiota
Poonam Phalak,Michael A. Henson +1 more
TL;DR: A multispecies biofilm model is developed by combining genome-scale metabolic reconstructions of C. difficile, Bacteroides thetaiotaomicron from the phylum Bacteroidetes, Faecalibacterium prausnitzii from thephylum Firmicutes, and Escherichia coli from thePhylum Proteobacteria that has the capability to support the development of new treatment strategies for C.difficile gut infections.
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Genetic Dissection of the Fermentative and Respiratory Contributions Supporting Vibrio cholerae Hypoxic Growth
Emilio Bueno,Brandon Sit,Brandon Sit,Matthew K. Waldor,Matthew K. Waldor,Matthew K. Waldor,Felipe Cava +6 more
TL;DR: The findings reveal the genetic basis of a specific interaction between disparate energy production pathways that supports pathogen fitness under shifting conditions and identify metabolic vulnerabilities that may be targetable for new antimicrobial agents in V. cholerae and related pathogens.
Journal ArticleDOI
Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization
Fardou H. Heida,Hermie J. M. Harmsen,Albertus Timmer,Elisabeth M. W. Kooi,Arend F. Bos,Jan B.F. Hulscher +5 more
TL;DR: Bacterial invasion of the intestinal wall is more present in most affected NEC tissue samples compared with least affected NEC samples or controls and Enterobacteriaceae are prevalent in advanced NEC.
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Nitrate exposure induces intestinal microbiota dysbiosis and metabolism disorder in Bufo gargarizans tadpoles.
TL;DR: Exposure to 20 and 100 mg/L NO3-N could induce deficient nutrient absorption in intestine, resulting in malnutrition of B. gargarizans tadpoles, according to the results of functional prediction.
References
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
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A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
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Diversity of the human intestinal microbial flora.
Paul B. Eckburg,Elisabeth M. Bik,Charles N. Bernstein,Elizabeth Purdom,Les Dethlefsen,Michael Sargent,Steven R. Gill,Karen E. Nelson,David A. Relman,David A. Relman,David A. Relman +10 more
TL;DR: A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms, and significant intersubject variability and differences between stool and mucosa community composition were discovered.
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Obesity alters gut microbial ecology
Ruth E. Ley,Fredrik Bäckhed,Peter J. Turnbaugh,Catherine A. Lozupone,Robin D. Knight,Jeffrey I. Gordon +5 more
TL;DR: Analysis of the microbiota of genetically obese ob/ob mice, lean ob/+ and wild-type siblings, and their ob/+ mothers, all fed the same polysaccharide-rich diet, indicates that obesity affects the diversity of the gut microbiota and suggests that intentional manipulation of community structure may be useful for regulating energy balance in obese individuals.
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Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases
Daniel N. Frank,Allison L. St. Amand,Robert A. Feldman,Edgar C. Boedeker,Noam Harpaz,Norman R. Pace +5 more
TL;DR: Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances.