Open Access
Supplementary Materials for Host-Derived Nitrate Boosts Growth of E. coli in the Inflamed Gut
Sebastian E. Winter,Maria G. Winter,Mariana N. Xavier,A. Marijke Keestra,Richard Laughlin,Gabriel Gomez,Jing Wu,Ina Popova,Sanjai J. Parikh,L. Garry Adams,Renée M. Tsolis,Andreas J. Bäumler +11 more
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The article was published on 2013-01-01 and is currently open access. It has received 505 citations till now.read more
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Dysbiosis-Associated Change in Host Metabolism Generates Lactate to Support Salmonella Growth.
Caroline C. Gillis,Elizabeth R. Hughes,Luisella Spiga,Maria G. Winter,Wenhan Zhu,Tatiane Furtado de Carvalho,Rachael B. Chanin,Cassie L. Behrendt,Lora V. Hooper,Renato L. Santos,Sebastian E. Winter +10 more
TL;DR: It is concluded that alterations of the gut microbiota, specifically a depletion of Clostridia, reprogram host metabolism to perform lactate fermentation, thus supporting Salmonella infection.
Journal ArticleDOI
Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation
Kristina N.-M. Daeffler,Jeffrey D. Galley,Ravi U. Sheth,Laura Ortiz-Velez,Christopher O Bibb,Noah F. Shroyer,Robert A. Britton,Jeffrey J. Tabor +7 more
TL;DR: This work computationally identifies the first biological thiosulfate sensor and an improved tetrathionate sensor, both two‐component systems from marine Shewanella species, and validate them in laboratory Escherichia coli, and develops a method based upon oral gavage and flow cytometry of colon and fecal samples to demonstrate that colon inflammation activates the thios sulfurate sensor in mice harboring native gut microbiota.
Journal ArticleDOI
Streptomycin-Induced Inflammation Enhances Escherichia coli Gut Colonization Through Nitrate Respiration
Alanna M. Spees,Tamding Wangdi,Christopher A. Lopez,Dawn D. Kingsbury,Mariana N. Xavier,Sebastian E. Winter,Renée M. Tsolis,Andreas J. Bäumler +7 more
TL;DR: It is shown that streptomycin treatment rendered mice more susceptible to the development of chemically induced colitis, raising the possibility that the antibiotic might lower colonization resistance by changing mucosal immune responses rather than by preventing microbe-microbe interactions.
Journal ArticleDOI
Associations between host gene expression, the mucosal microbiome, and clinical outcome in the pelvic pouch of patients with inflammatory bowel disease
Xochitl C. Morgan,Xochitl C. Morgan,Boyko Kabakchiev,Levi Waldron,Levi Waldron,Andrea D. Tyler,Timothy L. Tickle,Timothy L. Tickle,Raquel Milgrom,Joanne M. Stempak,Dirk Gevers,Ramnik J. Xavier,Mark S. Silverberg,Curtis Huttenhower,Curtis Huttenhower +14 more
TL;DR: This study quantifies the effects of inflammation, antibiotic use, and biopsy location upon the microbiome and host transcriptome during pouchitis, and suggests that cross-sectional changes in gut epithelial transcription are not a major component of the host-microbiome regulatory interfaceDuring pouchitis.
Journal Article
Eubiosis and dysbiosis: the two sides of the microbiota
Valerio Iebba,Valentina Totino,Antonella Gagliardi,Floriana Santangelo,Fatima Cacciotti,Maria Trancassini,Carlo Mancini,Clelia Cicerone,Enrico Corazziari,Fabrizio Pantanella,Serena Schippa +10 more
TL;DR: The microbial ecosystem of the gastrointestinal tract is characterized by a great number of microbial species living in balance by adopting mutualistic strategies, and eubiosis and dysbiosis status: the two sides of the microbiota.
References
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
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TL;DR: A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms, and significant intersubject variability and differences between stool and mucosa community composition were discovered.
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Obesity alters gut microbial ecology
Ruth E. Ley,Fredrik Bäckhed,Peter J. Turnbaugh,Catherine A. Lozupone,Robin D. Knight,Jeffrey I. Gordon +5 more
TL;DR: Analysis of the microbiota of genetically obese ob/ob mice, lean ob/+ and wild-type siblings, and their ob/+ mothers, all fed the same polysaccharide-rich diet, indicates that obesity affects the diversity of the gut microbiota and suggests that intentional manipulation of community structure may be useful for regulating energy balance in obese individuals.
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Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases
Daniel N. Frank,Allison L. St. Amand,Robert A. Feldman,Edgar C. Boedeker,Noam Harpaz,Norman R. Pace +5 more
TL;DR: Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances.