Virus-helminth coinfection reveals a microbiota-independent mechanism of immunomodulation
Lisa C. Osborne,Laurel A. Monticelli,Timothy J. Nice,Tara E. Sutherland,Mark C. Siracusa,Matthew R. Hepworth,Vesselin T. Tomov,Dmytro Kobuley,Sara Tran,Kyle Bittinger,Aubrey Bailey,Alice Laughlin,Jean-Luc Boucher,E. John Wherry,Frederic D. Bushman,Judith E. Allen,Herbert W. Virgin,David Artis +17 more
TLDR
Data indicate that helminth-induced immunomodulation occurs independently of changes in the microbiota but is dependent on Ym1, a chitinase-like molecule that is associated with alternatively activated macrophages, which could partially restore antiviral immunity.Abstract:
The mammalian intestine is colonized by beneficial commensal bacteria and is a site of infection by pathogens, including helminth parasites. Helminths induce potent immunomodulatory effects, but whether these effects are mediated by direct regulation of host immunity or indirectly through eliciting changes in the microbiota is unknown. We tested this in the context of virus-helminth coinfection. Helminth coinfection resulted in impaired antiviral immunity and was associated with changes in the microbiota and STAT6-dependent helminth-induced alternative activation of macrophages. Notably, helminth-induced impairment of antiviral immunity was evident in germ-free mice, but neutralization of Ym1, a chitinase-like molecule that is associated with alternatively activated macrophages, could partially restore antiviral immunity. These data indicate that helminth-induced immunomodulation occurs independently of changes in the microbiota but is dependent on Ym1.read more
Citations
More filters
Journal ArticleDOI
Impact of Helminth Infections on Female Reproductive Health and Associated Diseases.
Alisha Chetty,Millicent A. Omondi,Claire Butters,Katherine A. Smith,Katherine A. Smith,Gnatoulma Katawa,Manuel Ritter,Laura E. Layland,William G. C. Horsnell,William G. C. Horsnell +9 more
TL;DR: Current knowledge about the direct and systemic effects of helminth infections on unrelated diseases is explored and limited knowledge of how helminths infections directly cause pathology to female reproductive tract (FRT), alter susceptibility to sexually transmitted infections and reproduction is introduced.
Journal ArticleDOI
Intestinal fluke Metagonimus yokogawai infection increases probiotic Lactobacillus in mouse cecum
Ju Yeong Kim,Eun Min Kim,Myung Hee Yi,Jin-Young Lee,Seogwon Lee,Younjee Hwang,Dongeun Yong,Woon Mok Sohn,Tai Soon Yong +8 more
TL;DR: Although there was no apparent difference in species richness and diversity, the microbiome composition was different in the infected and control groups, and several Lactobacillus species with known immunomodulatory role in immune-mediated diseases were increased in theinfected group.
Journal ArticleDOI
β-Glucan-triggered Akkermansia muciniphila expansion facilitates the expulsion of intestinal helminth via TLR2 in mice.
TL;DR: This paper showed that β-glucans (BG) could trigger worm expulsion via mucus layer independently of type 2 immunity, but was dependent on the gut microbiota in mice, and daily oral supplementation of pasteurized A. muciniphila has a stronger deworming effect than live bacteria and interacted with TLR2.
Journal ArticleDOI
Gnotobiotic mouse model's contribution to understanding host-pathogen interactions.
Klara Kubelkova,Milota Benuchova,Hana Kozakova,Marek Sinkora,Zuzana Krocova,Jaroslav Pejchal,Ales Macela +6 more
TL;DR: This brief review is dedicated to the legacy of Prof. Jaroslav Šterzl and his colleagues, who laid the foundation for gnotobiology in the former Czechoslovakia 55 years, which represented a true milestone in the development of not solely Czechoslovak but also European and global immunology.
Journal ArticleDOI
Helminth-virus interactions: determinants of coinfection outcomes.
TL;DR: In this article, the authors studied the effects of coinfections in model mammalian organisms under specific pathogen-free conditions and found that infection with one organism can alter host host health.
References
More filters
Journal ArticleDOI
QIIME allows analysis of high-throughput community sequencing data.
J. Gregory Caporaso,Justin Kuczynski,Jesse Stombaugh,Kyle Bittinger,Frederic D. Bushman,Elizabeth K. Costello,Noah Fierer,Antonio Gonzalez Peña,Julia K. Goodrich,Jeffrey I. Gordon,Gavin A. Huttley,Scott T. Kelley,Dan Knights,Jeremy E. Koenig,Ruth E. Ley,Catherine A. Lozupone,Daniel McDonald,Brian D. Muegge,Meg Pirrung,Jens Reeder,Joel Sevinsky,Peter J. Turnbaugh,William A. Walters,Jeremy Widmann,Tanya Yatsunenko,Jesse R. Zaneveld,Rob Knight,Rob Knight +27 more
TL;DR: An overview of the analysis pipeline and links to raw data and processed output from the runs with and without denoising are provided.
Journal ArticleDOI
Search and clustering orders of magnitude faster than BLAST
TL;DR: UCLUST is a new clustering method that exploits USEARCH to assign sequences to clusters and offers several advantages over the widely used program CD-HIT, including higher speed, lower memory use, improved sensitivity, clustering at lower identities and classification of much larger datasets.
vegan: Community Ecology Package
Jari Oksanen,F. Guillaume Blanchet,Roeland Kindt,Pierre Legendre,Peter R. Minchin,Robert B. O'Hara,Gavin Simpson,Péter Sólymos,M. Henry H. Stevens,Helene H. Wagner +9 more
Journal ArticleDOI
APE: Analyses of Phylogenetics and Evolution in R language
TL;DR: UNLABELLED Analysis of Phylogenetics and Evolution (APE) is a package written in the R language for use in molecular evolution and phylogenetics that provides both utility functions for reading and writing data and manipulating phylogenetic trees.
Journal ArticleDOI
FastTree 2--approximately maximum-likelihood trees for large alignments.
TL;DR: Improvements to FastTree are described that improve its accuracy without sacrificing scalability, and FastTree 2 allows the inference of maximum-likelihood phylogenies for huge alignments.
Related Papers (5)
The Intestinal Microbiota Contributes to the Ability of Helminths to Modulate Allergic Inflammation
Mario M. Zaiss,Alexis Rapin,Luc Lebon,Lalit Kumar Dubey,Ilaria Mosconi,Kerstin Sarter,Alessandra Piersigilli,Alessandra Piersigilli,Laure Menin,Alan W. Walker,Alan W. Walker,Jacques Rougemont,Oonagh Paerewijck,Peter Geldhof,Kathleen McCoy,Andrew J. Macpherson,John Croese,Paul R. Giacomin,Alex Loukas,Tobias Junt,Benjamin J. Marsland,Nicola L. Harris +21 more