Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit.
Arun K. Nalla,Amanda M. Casto,Amanda M. Casto,Meei Li W. Huang,Garrett A. Perchetti,Reigran Sampoleo,Lasata Shrestha,Yulun Wei,Haiying Zhu,Keith R. Jerome,Keith R. Jerome,Alexander L. Greninger +11 more
TLDR
All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in the authors' analyses regardless of the primer-probe set or kit used.Abstract:
Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Washington. Given the rapid increase in the number of cases in many localities, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis. In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we evaluated assays using seven different primer-probe sets and one assay kit. We found that the most sensitive assays were those that used the E-gene primer-probe set described by Corman et al. (V. M. Corman, O. Landt, M. Kaiser, R. Molenkamp, et al., Euro Surveill 25:2000045, 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set developed by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020, https://www.cdc.gov/coronavirus/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf). All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer-probe set or kit used. These results will provide valuable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.read more
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First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community.
Warish Ahmed,Nicola Angel,Janette Edson,Kyle Bibby,Aaron Bivins,Jake W. O'Brien,Phil M. Choi,Masaaki Kitajima,Stuart L. Simpson,Jiaying Li,Benjamin J. Tscharke,Rory Verhagen,Wendy Smith,Julian Zaugg,Leanne Dierens,Philip Hugenholtz,Kevin V. Thomas,Jochen F. Mueller +17 more
TL;DR: The quantification of SARS-CoV-2 in wastewater affords the ability to monitor the prevalence of infections among the population via wastewater-based epidemiology (WBE) and highlights the viability of WBE for monitoring infectious diseases, such as COVID-19, in communities.
Journal ArticleDOI
Interpreting Diagnostic Tests for SARS-CoV-2.
TL;DR: How to interpret 2 types of diagnostic tests commonly in use for SARS-CoV-2 infections—reverse transcriptase–polymerase chain reaction (RT-PCR) and IgM and IgG enzyme-linked immunosorbent assay (ELISA)—and how the results may vary over time is described.
Journal ArticleDOI
SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology.
Flávio P. Veras,Marjorie Cornejo Pontelli,Camila Meirelles de Souza Silva,Juliana E Toller-Kawahisa,Mikhael de Lima,Daniele C. Nascimento,Ayda Henriques Schneider,Diego B. Caetite,Lucas Alves Tavares,Isadora Marques Paiva,Roberta Ribeiro Costa Rosales,David F. Colón,Ronaldo B. Martins,Italo A. Castro,Glaucia M. Almeida,Maria Isabel Fernandes Lopes,Maira N. Benatti,Letícia Pastorelli Bonjorno,Marcela C Giannini,Rodrigo Luppino-Assad,Sérgio C. L. Almeida,Fernando Crivelenti Vilar,Rodrigo de Carvalho Santana,Valdes Roberto Bollela,Maria Auxiliadora-Martins,Marcos C. Borges,Carlos Henrique Miranda,Antonio Pazin-Filho,Luis Lamberti Pinto da Silva,Larissa D. Cunha,Dario S. Zamboni,Felipe Dal-Pizzol,Luiz O. Leiria,Li Siyuan,Sabrina Setembre Batah,Alexandre Todorovic Fabro,Thais Mauad,Marisa Dolhnikoff,Amaro Nunes Duarte-Neto,Paulo Hilário Nascimento Saldiva,Thiago M. Cunha,José C. Alves-Filho,Eurico Arruda,Paulo Louzada-Junior,Renê Donizeti Ribeiro de Oliveira,Fernando Q. Cunha +45 more
TL;DR: It is described that SARS-CoV-2 triggers the release of ACE2-depended neutrophil extracellular traps (NETs) that mediate lung pathology, supporting the use of NETs inhibitors for COVID-19 treatment.
Journal ArticleDOI
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets.
Chantal B.F. Vogels,Anderson F. Brito,Anne L. Wyllie,Joseph R. Fauver,Isabel M. Ott,Chaney C. Kalinich,Mary E. Petrone,Arnau Casanovas-Massana,M. Catherine Muenker,Adam J. Moore,Jonathan Klein,Peiwen Lu,Alice Lu-Culligan,Xiaodong Jiang,Daniel J. Kim,Eriko Kudo,Tianyang Mao,Miyu Moriyama,Ji Eun Oh,Annsea Park,Julio Silva,Eric Song,Takehiro Takahashi,Manabu Taura,Maria Tokuyama,Arvind Venkataraman,Orr-El Weizman,Patrick Wong,Yexin Yang,Nagarjuna R. Cheemarla,Elizabeth B. White,Sarah Lapidus,Rebecca Earnest,Bertie Geng,Pavithra Vijayakumar,Camila D. Odio,John Fournier,Santos Bermejo,Shelli F. Farhadian,Charles S. Dela Cruz,Akiko Iwasaki,Akiko Iwasaki,Albert I. Ko,Marie L. Landry,Ellen F. Foxman,Nathan D. Grubaugh +45 more
TL;DR: Comparisons of RT–qPCR analytical efficiency and sensitivity show that all primer–probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction.
Journal ArticleDOI
SARS-CoV-2 in wastewater: State of the knowledge and research needs.
Masaaki Kitajima,Warish Ahmed,Kyle Bibby,Annalaura Carducci,Charles P. Gerba,Kerry A. Hamilton,Eiji Haramoto,Joan B. Rose +7 more
TL;DR: There is an urgent need for further research to establish methodologies for wastewater surveillance and understand the implications of the presence of SARS-CoV-2 in wastewater.
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