DADA2: High-resolution sample inference from Illumina amplicon data
Benjamin J. Callahan,Paul J. McMurdie,Michael J. Rosen,Andrew W. Han,Amy Jo A. Johnson,Susan Holmes +5 more
TLDR
The open-source software package DADA2 for modeling and correcting Illumina-sequenced amplicon errors is presented, revealing a diversity of previously undetected Lactobacillus crispatus variants.Abstract:
We present the open-source software package DADA2 for modeling and correcting Illumina-sequenced amplicon errors (https://github.com/benjjneb/dada2). DADA2 infers sample sequences exactly and resolves differences of as little as 1 nucleotide. In several mock communities, DADA2 identified more real variants and output fewer spurious sequences than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant women, revealing a diversity of previously undetected Lactobacillus crispatus variants.read more
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Resilient microorganisms in dust samples of the International Space Station—survival of the adaptation specialists
Maximilian Mora,A. Perras,A. Perras,T. A. Alekhova,Lisa Wink,Robert Krause,Alina Aleksandrova,Tatiana Novozhilova,Christine Moissl-Eichinger +8 more
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Fungal Traits Important for Soil Aggregation.
Anika Lehmann,Weishuang Zheng,Masahiro Ryo,Katharina Soutschek,Julien Roy,Rebecca Rongstock,Stefanie Maaß,Matthias C. Rillig +7 more
TL;DR: The results point to a typical suite of traits characterizing fungi that are good soil aggregators, and the power of employing a trait-based approach to unravel biological mechanisms underpinning soil aggregation could now be extended also to other soil biota groups.
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Metabarcoding free‐living marine nematodes using curated 18S and CO1 reference sequence databases for species‐level taxonomic assignments
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Tools for Analysis of the Microbiome.
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TL;DR: The Human Microbiome Project Consortium reported the first results of their analysis of microbial communities from distinct, clinically relevant body habitats in a human cohort; the insights into the microbial communities of a healthy population lay foundations for future exploration of the epidemiology, ecology and translational applications of the human microbiome as discussed by the authors.
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