Journal ArticleDOI
Derivation and characterization of two sibling human embryonic stem cell lines from discarded grade III embryos.
TLDR
Two new hES cell lines are derived from the inner cell mass (ICM) of discarded grade III human embryos that were not suitable for in vitro fertility treatment, and they show an undifferentiated phenotype in culture for more than 65 passages and express pluripotency markers.Abstract:
Human embryonic stem (hES) cells are a valuable tool for studying human development in addition to their potential applications in regenerative medicine and drug discovery. The role of genetic background and epigenetic influences in development as well as in response to external influences such as drugs and therapies is well recognized. The great ethnic diversity in the Indian subcontinent translates to interindividual variability in drug response and disease susceptibility. For these reasons, new hES cell lines representing Indian genetic diversity will be valuable in studies of tissue-differentiation, cellular-function and for aspects of characterization of responses to drugs. We have derived two new hES cell lines, BJNhem19 and BJNhem20 from the inner cell mass (ICM) of discarded grade III human embryos that were not suitable for in vitro fertility treatment. Human leukocyte antigen (HLA) isotype analysis shows that they are genetically distinct from existing hES cell lines. Short tandem repeat (STR) analysis shows that the two cell lines are derived from sibling embryos. These cell lines show an undifferentiated phenotype in culture for more than 65 passages, show normal karyotype and express pluripotency markers such as TRA-1-60, TRA-1-81, stage-specific embryonic antigen-4 (SSEA-4), alkaline phosphatase, DNMT3B, GABRB3, GDF3, OCT4, NANOG, SOX2, TERF1, TDGF, LEFTA, THY1, and REX1. While both cell lines can differentiate into derivatives of all three germ layers in vitro, only BJNhem20 can form teratomas when transplanted into mice. We observe an increased frequency of cardiomyocyte differentiation from BJNhem20 embryoid bodies in feeder-free cultures upon induction with DMSO. Cardiomyocytes purified from such cultures survive and show rhythmic contractions for several weeks in culture. These hES cell lines have been accepted for deposit in the U.K. Stem Cell Bank and will be a useful resource for the international stem cell community.read more
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Patent
Differentiation of human embryonic stem cells
TL;DR: In this paper, a method for promoting the differentiation of pluripotent stem cells into insulin-producing cells was proposed, which is more than 80% of the cells within the population expressing characteristic markers of endodermal complete system.
Journal ArticleDOI
Diverse effects of dimethyl sulfoxide (DMSO) on the differentiation potential of human embryonic stem cells
TL;DR: It is found that hESC-based assays can provide timely alerts about the outcome of widespread applications of DMSO as drug solvent, cryoprotectant and differentiating agent in a dose-dependent manner.
Journal ArticleDOI
Human embryonic mesodermal progenitors highly resemble human mesenchymal stem cells and display high potential for tissue engineering applications.
Giuseppe Maria de Peppo,Sara Svensson,Maria Lennerås,Jane Synnergren,Jane Synnergren,Johan Stenberg,Raimund Strehl,Johan Hyllner,Peter Thomsen,Camilla Karlsson +9 more
TL;DR: The hES-MPs are thus an appealing alternative to hMSCs in tissue engineering applications and stem-cell-based therapies for mesodermal tissues and potentially used for HLA-incompatible transplantation.
Journal ArticleDOI
Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos
Svetlana Gavrilov,Darja Marolt,Nataki C. Douglas,Robert W. Prosser,Imran Khalid,Mark V. Sauer,Donald W. Landry,Gordana Vunjak-Novakovic,Virginia E. Papaioannou +8 more
TL;DR: Criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos.
Journal ArticleDOI
Generating minicorneal organoids from human induced pluripotent stem cells
Praveen Joseph Susaimanickam,Savitri Maddileti,Vinay Kumar Pulimamidi,Sreedhar Rao Boyinpally,Ramavat Ravinder Naik,Milind N. Naik,Geereddy Bhanuprakash Reddy,Virender S Sangwan,Indumathi Mariappan +8 more
TL;DR: An efficient method to generate complex three-dimensional corneal organoids from human PSCs is reported, which offer an alternative tissue source for regenerating different layers of the cornea and eliminate the need for complicated cell enrichment procedures.
References
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Journal ArticleDOI
Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes.
Izhak Kehat,Dorit Kenyagin-Karsenti,Mirit Snir,Hana Segev,Michal Amit,Amira Gepstein,Erella Livne,Ofer Binah,Joseph Itskovitz-Eldor,Lior Gepstein +9 more
TL;DR: The human ES cell--derived cardiomyocytes displayed structural and functional properties of early-stage cardiomers, which may have significant impact on the study of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.
Journal ArticleDOI
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
Oluseun Adewumi,Behrouz Aflatoonian,Lars Ährlund-Richter,Michal Amit,Peter W. Andrews,Gemma Beighton,Paul Bello,Nissim Benvenisty,Lorraine S. Berry,Simon Bevan,Barak Blum,Justin Brooking,Kevin G. Chen,Andre Bh Choo,Gary A. Churchill,Marie Corbel,Ivan Damjanov,John S Draper,Petr Dvorak,Petr Dvorak,Katarina Emanuelsson,Roland A. Fleck,Angela Ford,Karin Astrid Maria Gertow,Karin Astrid Maria Gertow,Marina Gertsenstein,Paul J. Gokhale,Rebecca S. Hamilton,Alex Hampl,Alex Hampl,Lyn Healy,Outi Hovatta,Johan Hyllner,Marta P. Imreh,Marta P. Imreh,Joseph Itskovitz-Eldor,Jamie P. Jackson,Jackie Johnson,Mark Jones,Kehkooi Kee,Benjamin L. King,Barbara B. Knowles,Majlinda Lako,Franck Lebrin,Barbara S. Mallon,Daisy Manning,Yoav Mayshar,Ronald D.G. McKay,Anna E. Michalska,Milla Mikkola,Masha Mileikovsky,Stephen L. Minger,Harry Moore,Christine L. Mummery,Andras Nagy,Norio Nakatsuji,Carmel M. O’Brien,Steve Oh,Cia Olsson,Timo Otonkoski,Kye-Yoon Park,Robert Passier,Hema Patel,Minal Patel,Roger A. Pedersen,Martin F. Pera,Marian S Piekarczyk,Renee A. Reijo Pera,Benjamin Reubinoff,Allan J. Robins,Janet Rossant,Peter J. Rugg-Gunn,Peter J. Rugg-Gunn,Thomas C Schulz,Henrik Semb,Eric S Sherrer,Henrike Siemen,Glyn Stacey,Miodrag Stojkovic,Hirofumi Suemori,Jin P. Szatkiewicz,Tikva Turetsky,Timo Tuuri,Steineke van den Brink,Kristina Vintersten,Sanna Vuoristo,Dorien Ward,Thomas A Weaver,Lesley Young,Weidong Zhang +89 more
TL;DR: The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide and found that despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers ofhuman embryonic stem cells.
Journal ArticleDOI
Multilineage differentiation from human embryonic stem cell lines.
TL;DR: Because they have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, human ES cells could potentially provide an unlimited supply of tissue for human transplantation.
Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TL;DR: The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.
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