DNA sequencing with chain-terminating inhibitors
TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
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Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE.
TL;DR: Results show that molybdate transport in E. coli is regulated by ModE, which acts as a repressor when bound to moly bdate, and DNA mobility shift experiments confirmed that ModE binds to an oligonucleotide derived from the operator region of the modABCD operon.
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The Drosophila SRF homolog is expressed in a subset of tracheal cells and maps within a genomic region required for tracheal development.
Markus Affolter,Jacques Montagne,Uwe Walldorf,Jay C. Groppe,Urs Kloter,Marilina LaRosa,Walter J. Gehring +6 more
TL;DR: The Drosophila SRF homolog (DSRF) codes for a protein that displays 93% sequence identity with human SRF in the MADS domain, the region required for DNA binding, dimerization and interaction with accessory factors.
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Contributions to the Phylogeny of the Cyclophyllidea (Cestoda) Inferred from Mitochondrial 12S rDNA
TL;DR: A high degree of variation within the examined 304-bp fragment was observed between two isolates of Taenia taeniaeformis, supporting often discussed genetic heterogeneity within this species and indicating that this species is genetically homogenous.
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Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.
TL;DR: The enzyme NAD(P)H:flavin oxidoreductase (flavin reductase) catalyzes the reduction of soluble flavins by reduced pyridine nucleotides in Escherichia coli and is part of a multienzyme system that reduces the Fe(III) center of ribonucleotide reduct enzyme to Fe(II) and thereby sets the stage for the generation by dioxygen of a free tyrosyl radical required for enzyme activity.
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Cloning, genomic organization, and characterization of a human cholinephosphotransferase.
TL;DR: Both the hCPT1 and hCEPT1 enzymes were able to reconstitute the synthesis of PC in yeast to levels provided by the endogenous yeast cholinephosphotransferase; however, only hCEPT2-derived activity was able to complement the yeast CPT1 gene in its interaction with SEC14 and affect cell growth.
References
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A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
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A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
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Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.