DNA sequencing with chain-terminating inhibitors
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TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
Citations
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The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
Kazuo Shinozaki,Masaru Ohme,Minoru Tanaka,Tatsuya Wakasugi,Nobuaki Hayashida,Tohru Matsubayashi,N. Zaita,J. Chunwongse,Junichi Obokata,Kazuko Yamaguchi-Shinozaki,Chikara Ohto,Keita Torazawa,B. Y. Meng,Mamoru Sugita,Hiroshi Deno,T. Kamogashira,Kyoji Yamada,Jun Kusuda,F. Takaiwa,Akira Kato,N. Tohdoh,Hiroaki Shimada,Masahiro Sugiura +22 more
TL;DR: Five sequences coding for proteins homologous to components of the respiratory‐chain NADH dehydrogenase from human mitochondria have been found and sequence and expression analyses indicate both prokaryotic and eukaryotic features of the chloroplast genes.
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The impact of next-generation sequencing technology on genetics.
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Making antibody fragments using phage display libraries
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Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes
Axel Ullrich,John Richard Bell,Ellson Y. Chen,R. Herrera,L. M. Petruzzelli,Thomas J. Dull,Alane M. Gray,Lisa M. Coussens,Yu Cheng Liao,M. Tsubokawa,Anthony J. Mason,Peter H. Seeburg,C. Grunfeld,Ora M. Rosen,J. Ramachandran,J. Ramachandran +15 more
TL;DR: The entire 1,370-amino-acid sequence of the human insulin receptor precursor is deduced from a single complementary DNA clone, finding sequence homologies to human epidermal growth factor receptor and the members of the src family of oncogene products.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.