DNA sequencing with chain-terminating inhibitors
TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
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Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor.
Hiroshi Takeshima,Seiichiro Nishimura,Takeshi Matsumoto,Hiroyuki Ishida,Kenji Kangawa,Naoto Minamino,Hisayuki Matsuo,Masamichi Ueda,Masao Hanaoka,Tadaaki Hirose,Shosaku Numa +10 more
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Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily.
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A Family of Metabotropic Glutamate Receptors
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Mammalian multidrug resistance gene: Complete cDNA sequence indicates strong homology to bacterial transport proteins
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REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons
Jayhong A. Chong,José Tapia-Ramírez,Sandra Kim,Juan José Toledo-Aral,Yingcong Zheng,Michael Boutros,Yelena M. Altshuller,Michael A. Frohman,Susan D. Kraner,Gail Mandel +9 more
TL;DR: It is proposed that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.