DNA sequencing with chain-terminating inhibitors
TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
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Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.
Jörn Görlach,Sandra L. Volrath,Gertrud Knauf-Beiter,Georges Hengy,Uli Beckhove,Karl-Heinz Kogel,Michael Oostendorp,Theo Staub,Eric R. Ward,Helmut Kessmann,John Ryals +10 more
TL;DR: A novel synthetic chemical, benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH), was shown to induce acquired resistance in wheat and was the most potent inducer of both resistance and gene induction.
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Reverse transcription of R2Bm RNA is primed by a nick at the chromosomal target site: A mechanism for non-LTR retrotransposition
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Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist
Stephen P. Eisenberg,Ronald Evans,William P. Arend,Evie L. Verderber,Michael T Brewer,Hannum Charles H,Robert C. Thompson +6 more
TL;DR: Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity and analysis of monocyte RNA indicates that the gene is transcriptionally regulated.
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Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone-related peptide gene.
Andrew C. Karaplis,Arne Luz,Julia Glowacki,Roderick T. Bronson,Victor L. J. Tybulewicz,Henry M. Kronenberg,Richard C. Mulligan +6 more
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Production of human beta interferon in insect cells infected with a baculovirus expression vector.
TL;DR: Results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.