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DNA sequencing with chain-terminating inhibitors

Frederick Sanger, +2 more
- 01 Dec 1977 - 
- Vol. 74, Iss: 12, pp 5463-5467
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TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Abstract
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.

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Citations
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Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein

TL;DR: Primary rat embryo fibroblasts were transformed by a p53 mutant (alanine to valine change at amino acid 135) plus ras, and the S-phase cells appear to be immune to the p53 negative regulation of growth until they enter the next G1 period.
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Human Intestinal H+/Peptide Cotransporter: CLONING, FUNCTIONAL EXPRESSION, AND CHROMOSOMAL LOCALIZATION

TL;DR: Ch Chromosomal assignment studies with somatic cell hybrid analysis and in situ hybridization have located the gene encoding the cloned human H+/peptide cotransporter to chromosome 13 q33→q34.
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A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase.

TL;DR: Results indicate that this cDNA is the product of the human Lewis blood group locus, provide genetic confirmation of the hypothesis that this enzyme can catalyze two distinct transglycosylation reactions, and outline an approach to the isolation of other sequences that determine expression of developmentally regulated oligosaccharide antigens.
Journal ArticleDOI

Selecting high-affinity binding proteins by monovalent phage display.

TL;DR: Variants of human growth hormone (hGH) with increased affinity and specificity for the hGH receptor were isolated using an improved phage display system using alanine-scanning mutagenesis to modify and understand the energetics of protein-ligand interfaces.
Journal ArticleDOI

T cell receptor δ gene mutant mice : independent generation of αβ T cells and programmed rearrangements of γδ TCR genes

TL;DR: The analyses of TCR γ and δ genes in the mutant mice suggest that intracellular mechanisms acting at the level of DNA rearrangement play key roles in the differential δ and γ gene rearrangements and in the generation of the highly restricted junctional sequences during fetal thymic development.
References
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Journal ArticleDOI

A new method for sequencing DNA

TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI

A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI

Nucleotide sequence of bacteriophage G4 DNA.

TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.
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