DNA sequencing with chain-terminating inhibitors
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TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
Citations
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Cloning and expression of a cDNA encoding an endothelin receptor
TL;DR: The cloning of a complementary DNA encoding a bovine endothelin receptor is reported, which has a transmembrane topology similar to that of other G protein-coupled receptors and shows specific binding, with the highest selectivity to ET-1 in animal cells trans-fected with the cloned cDNA.
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Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.
TL;DR: The results suggest that activation of the PD‐1 gene may be involved in the classical type of programmed cell death.
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Humanization of an anti-p185HER2 antibody for human cancer therapy.
Paul Carter,L. G. Presta,Cornelia M. Gorman,John B. Ridgway,Dennis J. Henner,Wai Lee Wong,A. M. Rowland,Claire Kotts,M. E. Carver,H M Shepard +9 more
TL;DR: The murine monoclonal antibody mumAb4D5, directed against human epidermal growth factor receptor 2 (p 185HER2), specifically inhibits proliferation of human tumor cells overexpressing p185HER2, but the efficacy of mumAb 4D5 in human cancer therapy is likely to be limited by a human anti-mouse antibody response and lack of effector functions.
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Heteromeric NMDA receptors: Molecular and functional distinction of subtypes
Hannah Monyer,Rolf Sprengel,Ralf Schoepfer,Anne Herb,Miyoko Higuchi,Hilda Lomelí,Nail Burnashev,Bert Sakmann,Peter H. Seeburg +8 more
TL;DR: Molecular cloning identified three complementary DNA species of rat brain, encoding NMDA receptor subunits NMDAR2A (NR2A), NR2B, and NR2C, which are 55 to 70% ientical in sequence, and these are structurally related, with less than 20% sequence identity, to other excitatory amino acid receptor sub Units.
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The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes.
Akihiro Inoue,Masashi Yanagisawa,Sadao Kimura,Yoshitoshi Kasuya,Takashi Miyauchi,Katsutoshi Goto,Tomoh Masaki +6 more
TL;DR: Three distinct human endothelin-related genes were cloned by screening a genomic DNA library under a low hybridization stringency with a synthetic oligonucleotide probe encoding a portion of the endotheli sequence, suggesting the possible existence of endothelins receptor subtypes.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.