DNA sequencing with chain-terminating inhibitors
TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
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The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins
Patrick J. O'Hara,Paul O. Sheppard,Henning Thøgersen,Domenick Venezia,Betty A. Haldeman,Vicki McGrane,Khaled M. Houamed,Christian Thomsen,Teresa Gilbert,Eileen R. Mulvihill +9 more
TL;DR: Sensitive sequence analysis techniques indicate that the metabotropic receptor extracellular domain is similar to bacterial periplasmic amino acid binding proteins, and a structural model built using the observed similarity predicts a ligand-binding site, and mutants with conservative amino acid substitutions at this site are shown to have reduced ligand affinity.
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Expression cloning of the murine erythropoietin receptor.
TL;DR: Two independent cDNA clones encoding the erythropoietin receptor (EPO-R) were isolated from a pXM expression library made from uninduced murine erythroleukemia (MEL) cells and showed no similarities to known proteins or nucleic acid sequences in the data bases.
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The herbicide sensitivity gene CHL1 of arabidopsis encodes a nitrate-inducible nitrate transporter
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Journal ArticleDOI
Selective amplification and cloning of four new members of the G protein-coupled receptor family.
Frédérick Libert,Marc Parmentier,Anne Lefort,Christiane Dinsart,J. Van Sande,Carine Maenhaut,Marie-Jeanne Simons,Jacques Emile Dumont,Gilbert Vassart +8 more
TL;DR: An approach based on the polymerase chain reaction has been devised to clone new members of the family of genes encoding guanosine triphosphate-binding protein (G protein)-coupled receptors, suggesting that they are members of a new subfamily of receptors with a very short nonglycosylated amino-terminal extension.
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Mutations in the palmitoyl protein thioesterase gene causing infantile neuronal ceroid lipofuscinosis
Jouni Vesa,Hellsten E,Linda A. Verkruyse,Laura A. Camp,Juhani Rapola,Pirkko Santavuori,Sandra L. Hofmann,Leena Peltonen +7 more
TL;DR: The identification, by positional candidate methods, of defects in the palmitoyl-protein thioesterase gene in all 42 Finnish INCL patients and several non-Finnish patients is reported, which results in intracellular accumulation of the polypeptide and undetectable enzyme activity in the brain of patients.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.