DNA sequencing with chain-terminating inhibitors
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TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
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Isolation of a novel gene underlying batten disease, CLN3
Terry J. Lerner,Rose-Mary Boustany,John W. Anderson,K. L. D'arigo,Karen Schlumpf,Alan Buckler,James F. Gusella,Jonathan L. Haines +7 more
TL;DR: Two separate deletions and a point mutation altering a splice site in three unrelated families have confirmed the candidate gene that is disrupted by a 1 kb genomic deletion in all patients carrying the 56 chromosome as the CLN3 gene.
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Genetic transfer of a functional human interferon α receptor into mouse cells: Cloning and expression of its c-DNA
TL;DR: Mouse cells expressing the cDNA become sensitive to the antiviral activity of and express binding sites for human interferon a, demonstrating that the cloned cDNA encodes a functional human interFERon a receptor.
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Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus.
TL;DR: The cloning of this gene in Escherichia coli by using an inserted transposon (Tn551) as a cloning probe is described, and the cloned gene showed considerable variation with respect to different exoproteins and different host strains compared with the chromosomal agr determinant.
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The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
TL;DR: The cDNA cloning of the human oct-1 gene, which encodes Oct-1, is reported by screening lambda gt11 recombinant phage in situ for octamer motif-specific DNA binding by screening for beta-galactosidase-octamer-binding fusion protein with DNA-binding specificity indistinguishable from human HeLa cellOct-1 protein.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.