DNA sequencing with chain-terminating inhibitors
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TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.Abstract:
A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.read more
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Hybrid error correction and de novo assembly of single-molecule sequencing reads
Sergey Koren,Michael C. Schatz,Brian P. Walenz,Jeffrey Martin,Jason T. Howard,Ganeshkumar Ganapathy,Zhong Wang,David A. Rasko,W. Richard McCombie,Erich D. Jarvis,Adam M. Phillippy +10 more
TL;DR: This work introduces a correction algorithm and assembly strategy that uses short, high-fidelity sequences to correct the error in single-molecule sequences, leading to substantially better assemblies than current sequencing strategies.
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Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400.
Teiichi Furuichi,Shingo Yoshikawa,Shingo Yoshikawa,Atsushi Miyawaki,Atsushi Miyawaki,Kentaroh Wada,Nobuaki Maeda,Katsuhiko Mikoshiba,Katsuhiko Mikoshiba +8 more
TL;DR: Cloning and expression of functional P400 protein from cerebellar Purkinje neurons shows that this protein is a receptor for inositol 1,4,5-triphosphate, a second messenger that mediates the release of intracellular calcium.
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Identification of a retinoic acid responsive element in the retinoic acid receptor & beta;gene
TL;DR: This work has identified a 27-base pair fragment, located 59 base pairs upstream of the transcriptional start, which confers retinoic acid responsiveness on the herpes virus thymidine kinase promoter, which contains a perfect direct repeat of the motif GTTCAC, which is reminiscent of the 5′ half-palindrome of the thyroid and oestrogen hormone responsive elements.
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Next-generation DNA sequencing techniques.
TL;DR: Development of commercial sequencing devices is reviewed, some European contributions to the field are mentioned, and presently commercially available very high-throughput DNA sequencing platforms, as well as techniques under development, are described and their applications in bio-medical fields discussed.
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Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel
TL;DR: A complementary DNA clone containing the coding sequence of one of these rapidly responding channels, a 5HT3 subtype of the serotonin receptor, has been isolated by screening a neuroblastoma expression library for functional expression of serotonin-gated currents in Xenopus oocytes.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
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A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
Frederick Sanger,Alan Coulson +1 more
TL;DR: A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA.
Journal ArticleDOI
Nucleotide sequence of bacteriophage G4 DNA.
TL;DR: The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.