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GENEVESTIGATOR. Arabidopsis Microarray Database and Analysis Toolbox

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TLDR
Genevestigator as mentioned in this paper is a web-browser interface for gene expression analysis using Affymetrix GeneChip data, which allows users to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs.
Abstract
High-throughput gene expression analysis has become a frequent and powerful research tool in biology. At present, however, few software applications have been developed for biologists to query large microarray gene expression databases using a Web-browser interface. We present GENEVESTIGATOR, a database and Web-browser data mining interface for Affymetrix GeneChip data. Users can query the database to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs. Reversely, mining tools allow users to identify genes specifically expressed during selected stresses, growth stages, or in particular organs. Using GENEVESTIGATOR, the gene expression profiles of more than 22,000 Arabidopsis genes can be obtained, including those of 10,600 currently uncharacterized genes. The objective of this software application is to direct gene functional discovery and design of new experiments by providing plant biologists with contextual information on the expression of genes. The database and analysis toolbox is available as a community resource at https://www.genevestigator.ethz.ch.

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Plant peptides in signalling: looking for new partners

TL;DR: The genetic similarities between the IDA and CLV3 signalling systems are used to hypothesize that closely related peptide ligands are likely to interact with families of closely related RLKs and ligand-receptor systems can be identified to improve the understanding of developmental processes in plants.
Journal ArticleDOI

Pollen tube NAD(P)H oxidases act as a speed control to dampen growth rate oscillations during polarized cell growth.

TL;DR: By limiting pollen tube growth, ROS produced by NAD(P)H oxidases modulate the amplitude and frequency of pollen tube Growth rate oscillations, indicating that ROS influence K(+) homeostasis.
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Jasmonic acid perception by COI1 involves inositol polyphosphates in Arabidopsis thaliana.

TL;DR: The combined data from experiments using mutated COI1 variants, as well as yeast and Arabidopsis backgrounds altered in inositol polyphosphate metabolism, indicate that an inositl polyph phosphate, and probably InsP₅, contributes to COI 1 function.
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Differential Impact of Lipoxygenase 2 and Jasmonates on Natural and Stress-Induced Senescence in Arabidopsis

TL;DR: Lipoxygenase 2-RNAi lines and the allene oxid synthase-deficient mutant dde2 were less sensitive to Sorbitol than the wild type, indicating that oxylipins contribute to the response to sorbitol stress.
Journal ArticleDOI

Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5

TL;DR: Root uptake of all tested amino acids, except L-Asp, was significantly affected in double AAP5*LHT1 mutants, suggesting that these two transporters account for a major proportion of roots' uptake of amino acids at low concentrations.
References
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Journal ArticleDOI

Gene Ontology: tool for the unification of biology

TL;DR: The goal of the Gene Ontology Consortium is to produce a dynamic, controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing.
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Gene Expression Omnibus: NCBI gene expression and hybridization array data repository

TL;DR: The Gene Expression Omnibus (GEO) project was initiated in response to the growing demand for a public repository for high-throughput gene expression data and provides a flexible and open design that facilitates submission, storage and retrieval of heterogeneous data sets from high-power gene expression and genomic hybridization experiments.
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Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.

TL;DR: This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.
PatentDOI

Expression monitoring by hybridization to high density oligonucleotide arrays

TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
Journal ArticleDOI

Hierarchical Organization of Modularity in Metabolic Networks

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