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GENEVESTIGATOR. Arabidopsis Microarray Database and Analysis Toolbox

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TLDR
Genevestigator as mentioned in this paper is a web-browser interface for gene expression analysis using Affymetrix GeneChip data, which allows users to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs.
Abstract
High-throughput gene expression analysis has become a frequent and powerful research tool in biology. At present, however, few software applications have been developed for biologists to query large microarray gene expression databases using a Web-browser interface. We present GENEVESTIGATOR, a database and Web-browser data mining interface for Affymetrix GeneChip data. Users can query the database to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs. Reversely, mining tools allow users to identify genes specifically expressed during selected stresses, growth stages, or in particular organs. Using GENEVESTIGATOR, the gene expression profiles of more than 22,000 Arabidopsis genes can be obtained, including those of 10,600 currently uncharacterized genes. The objective of this software application is to direct gene functional discovery and design of new experiments by providing plant biologists with contextual information on the expression of genes. The database and analysis toolbox is available as a community resource at https://www.genevestigator.ethz.ch.

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Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

TL;DR: The intron-containing CDC20 gene copies provide conserved and redundant functions for cell cycle progression in plants and are required for meristem maintenance, plant growth and male gametophyte formation.
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Arabidopsis potential calcium sensors regulate nitric oxide levels and the transition to flowering.

TL;DR: CML23 and CML24 are potential calcium sensors that have partially overlapping function that may act to transduce calcium signals to regulate NO accumulation and NO levels influence the transition to flowering through both the photoperiod and autonomous regulatory pathways.
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Arabidopsis acyl-CoA-binding protein ACBP1 participates in the regulation of seed germination and seedling development.

TL;DR: It is demonstrated that ACBP1 promotes abscisic acid (ABA) signaling during germination and seedling development, and bimolecular fluorescence complementation assays indicated that AC BP1 interacts with PLDα1 at the plasma membrane.

Arabidopsis CHLI2 Can Substitute for CHLI1 1(C)(W)(OA)

TL;DR: It is shown that the chli1/chli1 chli2/chLI2 double knockout mutant was albino, and that both genes are required for optimal growth during de-etiolation.
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Flavin Nucleotide Metabolism in Plants : MONOFUNCTIONAL ENZYMES SYNTHESIZE FAD IN PLASTIDS

TL;DR: A model for subcellular distribution of enzymes that synthesize and hydrolyze flavin nucleotides in plants is proposed, suggesting that plants contain a type of FAD synthetase previously known to exist only in prokaryotes.
References
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Journal ArticleDOI

Gene Ontology: tool for the unification of biology

TL;DR: The goal of the Gene Ontology Consortium is to produce a dynamic, controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing.
Journal ArticleDOI

Gene Expression Omnibus: NCBI gene expression and hybridization array data repository

TL;DR: The Gene Expression Omnibus (GEO) project was initiated in response to the growing demand for a public repository for high-throughput gene expression data and provides a flexible and open design that facilitates submission, storage and retrieval of heterogeneous data sets from high-power gene expression and genomic hybridization experiments.
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Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.

TL;DR: This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.
PatentDOI

Expression monitoring by hybridization to high density oligonucleotide arrays

TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
Journal ArticleDOI

Hierarchical Organization of Modularity in Metabolic Networks

TL;DR: It is shown that the metabolic networks of 43 distinct organisms are organized into many small, highly connected topologic modules that combine in a hierarchical manner into larger, less cohesive units, with their number and degree of clustering following a power law.
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