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GENEVESTIGATOR. Arabidopsis Microarray Database and Analysis Toolbox

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TLDR
Genevestigator as mentioned in this paper is a web-browser interface for gene expression analysis using Affymetrix GeneChip data, which allows users to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs.
Abstract
High-throughput gene expression analysis has become a frequent and powerful research tool in biology. At present, however, few software applications have been developed for biologists to query large microarray gene expression databases using a Web-browser interface. We present GENEVESTIGATOR, a database and Web-browser data mining interface for Affymetrix GeneChip data. Users can query the database to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs. Reversely, mining tools allow users to identify genes specifically expressed during selected stresses, growth stages, or in particular organs. Using GENEVESTIGATOR, the gene expression profiles of more than 22,000 Arabidopsis genes can be obtained, including those of 10,600 currently uncharacterized genes. The objective of this software application is to direct gene functional discovery and design of new experiments by providing plant biologists with contextual information on the expression of genes. The database and analysis toolbox is available as a community resource at https://www.genevestigator.ethz.ch.

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Journal ArticleDOI

Functional analysis of transcription factors in Arabidopsis.

TL;DR: This mini-review presents an overview of Arabidopsis TFs and introduces strategies for the functional analysis of plant TFs, which include both traditional and recently developed technologies.
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Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions.

TL;DR: It is shown that methyl jasmonate treatment increases the de novo synthesis of ascorbic acid in Arabidopsis and tobacco Bright Yellow-2 (BY-2) suspension cells, the first report of a hormonal regulation of vitamin C biosynthesis in plants.
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The Ubiquitination Machinery of the Ubiquitin System

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ARABINAN DEFICIENT 1 Is a Putative Arabinosyltransferase Involved in Biosynthesis of Pectic Arabinan in Arabidopsis

TL;DR: Analysis of mutant lines with T-DNA insertion in the ARABINAN DEFICIENT 1 gene suggested that ARAD1 is an arabinan α-1,5-arabinosyltransferase, a putative glycosyl transferase of type 2 membrane protein with a signal anchor in Arabidopsis.
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The CCAAT binding factor can mediate interactions between CONSTANS‐like proteins and DNA

TL;DR: It is shown that the C-terminal plant-specific CCT domain of both COL proteins binds the trimeric CCAAT binding factor (CBF) via its HAP5/NF-YC component, which is likely to recruit COL proteins to their DNA target motifs in planta.
References
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Journal ArticleDOI

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