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GENEVESTIGATOR. Arabidopsis Microarray Database and Analysis Toolbox

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TLDR
Genevestigator as mentioned in this paper is a web-browser interface for gene expression analysis using Affymetrix GeneChip data, which allows users to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs.
Abstract
High-throughput gene expression analysis has become a frequent and powerful research tool in biology. At present, however, few software applications have been developed for biologists to query large microarray gene expression databases using a Web-browser interface. We present GENEVESTIGATOR, a database and Web-browser data mining interface for Affymetrix GeneChip data. Users can query the database to retrieve the expression patterns of individual genes throughout chosen environmental conditions, growth stages, or organs. Reversely, mining tools allow users to identify genes specifically expressed during selected stresses, growth stages, or in particular organs. Using GENEVESTIGATOR, the gene expression profiles of more than 22,000 Arabidopsis genes can be obtained, including those of 10,600 currently uncharacterized genes. The objective of this software application is to direct gene functional discovery and design of new experiments by providing plant biologists with contextual information on the expression of genes. The database and analysis toolbox is available as a community resource at https://www.genevestigator.ethz.ch.

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Manuscript in preparation

H Shimada
Journal ArticleDOI

Reactive oxygen species homeostasis and signalling during drought and salinity stresses

TL;DR: An overview of ROS homeostasis and signalling in response to drought and salt stresses is provided and the current understanding of ROS involvement in stress sensing, stress signalling and regulation of acclimation responses is discussed.
Journal ArticleDOI

Significance of Inducible Defense-related Proteins in Infected Plants

TL;DR: The evolutionary conservation of similar defense-related proteins in monocots and dicots, but also their divergent occurrence in other conditions, suggest that these proteins serve essential functions in plant life, whether in defense or not.
Journal ArticleDOI

Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis

TL;DR: Hundreds of Arabidopsis genes were found that outperform traditional reference genes in terms of expression stability throughout development and under a range of environmental conditions, and the developed PCR primers or hybridization probes for the novel reference genes will enable better normalization and quantification of transcript levels inArabidopsis in the future.
Journal ArticleDOI

The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions

Sabeeha S. Merchant, +118 more
- 12 Oct 2007 - 
TL;DR: Analyses of the Chlamydomonas genome advance the understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.
References
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Journal ArticleDOI

Transcriptional programs of early reproductive stages in Arabidopsis

TL;DR: This study analyzes the expression profile of more than 22,000 genes present on the Arabidopsis ATH1 microarray to identify genes with specific functions during reproduction and finds a significant enrichment of predicted secreted proteins smaller than 15 kD that could function directly as signaling molecules or as precursors for peptide hormones.
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An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins

TL;DR: An Arabidopsis thaliana cDNA clone encoding a 110 amino acid thylakoid protein has been sequenced as discussed by the authors, which has been shown to have similar properties to the high-light-inducible proteins (HLIPs) of cyanobacteria and red algae.
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Distinct roles for light-dependent NADPH:protochlorophyllide oxidoreductases (POR) A and B during greening in higher plants.

TL;DR: It is demonstrated that P OR A is required for greening and that the availability of POR A limits Chl accumulation during growth in cFR, and that POR B apparently provides a means to sustain light-dependent Chl biosynthesis in fully greened, mature plants in the absence of phototransformable Pchlide-F655.
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A Brassica napus gene family which shows sequence similarity to ascorbate oxidase is expressed in developing pollen. Molecular characterization and analysis of promoter activity in transgenic tobacco plants

TL;DR: The genomic clone named Bp10 contains a member of a small pollen-specific gene family of B. napus, which codes for proteins of 62 kDa showing approximately 30% sequence identify to cucumber and pumpkin ascorbate oxidases (AAOs).
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Arabidopsis transcript profiling on Affymetrix GeneChip arrays.

TL;DR: Overall, this study shows that results obtained with ATH1 and AG arrays are very comparable and hence that the analysis is largely independent of probe sets, however, the result emphasize the need for appropriate filtering schemes such as those based on the present and change calls provided by MAS5.0 rather than reliance solely on signal values.
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