Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Shifting paradigms: the seeds of oncogene addiction
TL;DR: Although the success of imatinib was not a revolution in the Copernican sense, it spawned a transformation in cancer research that has fueled an urgency to characterize cancer genomes comprehensively and discover the driver mutations in all cancers.
Book ChapterDOI
Implicating the bcr/abl gene in the pathogenesis of Philadelphia chromosome-positive human leukemia.
George Q. Daley,Yinon Ben-Neriah +1 more
TL;DR: The hypothesis that the Philadelphia chromosome might have a causative role in human chronic myelogenous leukemia (CML) was validated by the discovery that the human homologue of the v -abl oncogene of Abelson murine leukemia virus (A-MuLV) mapped to chromosome 9q34, precisely the locus disrupted in the formation of thePhiladelphia chromosome.
Journal ArticleDOI
Src-family kinases in the development and therapy of Philadelphia chromosome-positive chronic myeloid leukemia and acute lymphoblastic leukemia
TL;DR: The preclinical and clinical evidence demonstrating the involvement of SFKs in imatinib resistance and the progression of CML and Ph+ ALL are discussed, as well as the potential role of dual SFK/BCR-ABL inhibition in the management of these diseases.
Journal ArticleDOI
Clustering of aberrations to specific chromosomes in human neoplasms. II. A survey of 287 neoplasms.
Felix Mitelman,Göran Levan +1 more
Journal ArticleDOI
New agents in cancer clinical trials.
Julian Adams,Peter J. Elliott +1 more
TL;DR: This review attempts to highlight some of the new and exciting molecules representing previously unexplored targets which may add to the much needed armamentarium of cancer drugs.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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