Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Genomewide screening of DNA copy number changes in chronic myelogenous leukemia with the use of high‐resolution array‐based comparative genomic hybridization
Noriko Hosoya,Masashi Sanada,Yasuhito Nannya,Kumi Nakazaki,Lili Wang,Akira Hangaishi,Mineo Kurokawa,Shigeru Chiba,Seishi Ogawa +8 more
TL;DR: The data suggests that at least a proportion of CML patients carry still‐unknown cryptic genomic alterations that could affect a gene or genes of importance in the disease progression of C ML.
Journal ArticleDOI
An Overview and Update of Chronic Myeloid Leukemia for Primary Care Physicians
Austin T. Granatowicz,Caroline I. Piatek,Elizabeth Moschiano,Ihab El-Hemaidi,Joel D. Armitage,Mojtaba Akhtari +5 more
TL;DR: An overview of the clinical presentation, diagnostic approach, and treatment considerations of chronic myeloid leukemia is provided.
Journal ArticleDOI
Two complex translocations in chronic granulocytic leukemia involving chromosomes 22, 9, and a third chromosome
TL;DR: Several reports indicate that an abnormality of chromosome 9 is not essential for the development of Ph-positive CGL, but the very high frequency of its involvement suggests that some type of non-random somatic association may exist between 9q and 22q which makes simultaneous breakage likely.
Journal Article
Detection of BCR-ABL Proteins in Blood Cells of Benign Phase Chronic Myelogenous Leukemia Patients
TL;DR: A procedure involving Western blotting with an anti-ABL monoclonal antibody was developed that allows detection of P210 BCR-ABl and P145 ABL in cells from chronic phase and blast crisis CML patients, but as expected only P145ABL was found in normal white blood cells.
Journal ArticleDOI
How I treat Philadelphia chromosome–positive acute lymphoblastic leukemia
TL;DR: Increased understanding of the leukemic cell biology and pathogenesis will likely lead to the development of more effective agents, and regimens less reliant on chemotherapy, able to achieve deep levels of disease eradication.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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