Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Insights into the stem cells of chronic myeloid leukemia
TL;DR: The need for new agents that effectively and specifically target CML stem cells to produce non-toxic, but curative therapies that do not require lifelong treatments is emphasized.
Journal ArticleDOI
Cancer genetics: from Boveri and Mendel to microarrays
TL;DR: The human genome has now been sequenced, a century after the re-discovery of Mendel's Laws, and the publication of Theodor Boveri's chromosomal theory of heredity, to trace the historical landmarks of cancer genetics from these early days to the present time.
Journal ArticleDOI
Detection of Minimal Residual bcr/abl Transcripts by a Modified Polymerase Chain Reaction
Ming-Sheng Lee,Kun-Sang Chang,Emil J. Freireich,Hagop M. Kantarjian,Moshe Talpaz,Jose M. Trujillo,Sanford A. Stass +6 more
TL;DR: A combined method of S1 nuclease protection and polymerase chain reaction is used to amplified sequences representative of the chimeric bcr/abl transcripts, which have the potential to identify a subpopulation of Ph1-positive CML patients in remission who are at high risk of relapse.
Journal Article
Molecular cytogenetics: Rosetta stone for understanding cancer--twenty-ninth G. H. A. Clowes memorial award lecture.
TL;DR: This article provides me with an occasion to review the genetic changes that occur within the cancer cell that are critically involved in the transformation of a normal to a malignant cell and to limit my consideration to those changes that have been detected by analyzing the karyotypic pattern of human cancer cells using chromosome banding.
Book ChapterDOI
Genetics and Etiology of Human Cancer
TL;DR: One of the highest national priorities that has been established for biomedical science in the United States is the control of cancer, which will probably necessitate a better understanding of the etiology, pathogenesis, and pathophysiology of cancer than is current.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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