Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Significance of the P210 Versus P190 Molecular Abnormalities in Adults With Philadelphia Chromosome-Positive Acute Leukemia
Hagop M. Kantarjian,Moshe Talpaz,Kapil Dhingra,Elihu H. Estey,Michael J. Keating,Stella Ku,Jose M. Trujillo,Yang Huh,Sanford A. Stass,Razelle Kurzrock +9 more
TL;DR: It is concluded that the molecular studies in Ph-positive acute leukemia are not associated with significantly different clinico- laboratory, karyotypic, or prognostic implications.
Journal ArticleDOI
The Philadelphia chromosome in leukemogenesis
Zhi Jie Kang,Zhi Jie Kang,Yu Fei Liu,Yu Fei Liu,L. Xu,Zi Jie Long,Dan Huang,Ya Yang,Ya Yang,Bing Liu,Bing Liu,Jiu Xing Feng,Jiu Xing Feng,Yu Jia Pan,Yu Jia Pan,Jinsong Yan,Quentin Liu,Quentin Liu +17 more
TL;DR: An overview of the clinical presentation and cellular biology of different phenotypes of Ph-positive leukemia and key findings regarding leukemogenesis are provided.
Journal ArticleDOI
Dynamics of BCR-ABL mRNA expression in first-line therapy of chronic myelogenous leukemia patients with imatinib or interferon alpha/ara-C.
Martin C. Müller,Norbert Gattermann,T. Lahaye,Michael W. Deininger,A. Berndt,Stefan Fruehauf,Andreas Neubauer,Thomas Fischer,D. K. Hossfeld,F. Schneller,Stefan W. Krause,Christoph Nerl,Herbert G. Sayer,Oliver G. Ottmann,Cornelius F. Waller,Walter E. Aulitzky,P. le Coutre,Mathias Freund,Kirsten Merx,Peter Paschka,Heiko Konig,Sebastian Kreil,Ute Berger,Harald Gschaidmeier,Ruediger Hehlmann,Andreas Hochhaus +25 more
TL;DR: It is concluded that treatment with imatinib in newly diagnosed CML patients is associated with a rapid decrease of BCR-ABL transcript levels, and nested PCR may reveal residual BCR/ABL transcripts in samples that are negative by real-time PCR.
Journal ArticleDOI
Clinical implications of tumor-cell heterogeneity.
TL;DR: Morphologic heterogeneity that is characterized by nearly normal differentiation and marked anaplasia is often observed within a single neoplasm, and the biochemical properties of neoplastic cells within the same tumor may also vary.
Book ChapterDOI
The role of mechanistic factors in promoting chromosomal translocations found in lymphoid and other cancers.
Yu Zhang,Monica Gostissa,Dominic G. Hildebrand,Michael S. Becker,Cristian Boboila,Roberto Chiarle,Susanna M. Lewis,Frederick W. Alt +7 more
TL;DR: Recent findings on processes and pathways that influence the initiation of chromosomal translocations, including the generation of DNA double strand breaks (DSBs) by general factors or in the context of the Lymphocyte-specific V(D)J and IgH class-switch recombination processes are discussed.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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