Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Significance of non-standard Philadelphia chromosomes in chronic granulocytic leukaemia.
TL;DR: It is concluded that the myeloid karyotype can provide prognostic as well as diagnostic information in patients with CGL and that the non-standard PhI chromosomes were simply occurring in older patients or were affecting prognosis independently.
Journal ArticleDOI
Evaluation of antileukaemic effects of rapamycin in patients with imatinib-resistant chronic myeloid leukaemia.
Christian Sillaber,Matthias Mayerhofer,Alexandra Böhm,A. Vales,Alexander Gruze,Karl J. Aichberger,Harald Esterbauer,Michael Pfeilstöcker,W. R. Sperr,Winfried F. Pickl,Oskar A. Haas,Peter Valent +11 more
TL;DR: This data indicates that the mammalian target of rapamycin (mTOR) is involved in the regulation of growth of neoplastic cells in chronic myeloid leukaemia (CML).
Journal ArticleDOI
Flow Cytometric Measurement of Blood Cells With BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
Liza Löf,Linda Arngården,Ulla Olsson-Strömberg,Benjamin Siart,Mattias Jansson,Joakim S. Dahlin,Ingrid Thörn,Lisa Christiansson,Monica Hermansson,Anders Larsson,Erik Ahlstrand,Göran Wålinder,Ola Söderberg,Richard Rosenquist,Ulf Landegren,Masood Kamali-Moghaddam +15 more
TL;DR: This flow cytometry-based method can serve as an attractive approach for routine measurement of cells harboring BCR-ABL1 fusions, also allowing simultaneously assessment of other cell surface markers as well as sensitive longitudinal follow-up.
Journal ArticleDOI
Variant Ph translocations in chronic myeloid leukemia
TL;DR: A review of the chromosomal breakpoints involved in this series and in 185 cases of variant Ph translocations previously reported in the literature reveals that a disproportionately large number of breakpoints are located in light-staining regions of G-banded chromosomes.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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