Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
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The promise of molecular targeted therapies: Protein kinase inhibitors in the treatment of cutaneous malignancies
TL;DR: A critical review of the literature is performed, highlighting selective inhibitors of signal transduction molecules involved in nonmelanoma skin cancer, melanoma, dermatofibrosarcoma protuberans, Merkel cell carcinoma, Kaposi's sarcoma, and systemic mastocytosis as discussed by the authors.
Journal ArticleDOI
Emerging Therapeutic Strategies for Targeting Chronic Myeloid Leukemia Stem Cells
TL;DR: Recent research done on alternative targeted therapies with a particular focus on some important signaling pathways that have the potential to target CML stem cells and potentially provide cure for CML are summarized.
Journal ArticleDOI
Identification of CD25 as STAT5-Dependent Growth Regulator of Leukemic Stem Cells in Ph+ CML
Irina Sadovnik,Andrea Hoelbl-Kovacic,Harald Herrmann,Gregor Eisenwort,Sabine Cerny-Reiterer,Wolfgang Warsch,Wolfgang Warsch,Gregor Hoermann,Georg Greiner,Katharina Blatt,Barbara Peter,Gabriele Stefanzl,Daniela Berger,Martin Bilban,Susanne Herndlhofer,Heinz Sill,Wolfgang R. Sperr,Berthold Streubel,Christine Mannhalter,Tessa L. Holyoake,Veronika Sexl,Peter Valent +21 more
TL;DR: CD25 is a novel STAT5-dependent marker of CML LSCs and may be useful for LSC detection and LSC isolation in clinical practice and basic science and may pave the way for the development of new more effective LSC-eradicating treatment strategies in CML.
Journal ArticleDOI
Karyotype patterns in human meningiomas. A comparison between studies with G- and Q-banding techniques
TL;DR: The results agreed with previous studies using the Q-banding technique and indicated a specific pattern of the G group autosomes in meningiomas.
Journal ArticleDOI
Bcr-Abl Regulates Protein Kinase Cι (PKCι) Transcription via an Elk1 Site in the PKCι Promoter
W. Clay Gustafson,Sutapa Ray,Lee Jamieson,E. Aubrey Thompson,Allan R. Brasier,Alan P. Fields +5 more
TL;DR: In this paper, the authors showed that Bcr-Abl-dependent activation of an Elk1 element within the proximal protein kinase C (PKC) isozyme promoter is required for BcrAbl mediated resistance of human K562 chronic myelogenous leukemia (CML) cells to Taxol-induced apoptosis.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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