Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Philadelphia chromosome positive myelodysplastic syndrome and acute myeloid leukemia-retrospective study and review of literature.
Yi-Kong Keung,Michael W. Beaty,Bayard L. Powell,Istvan Molnar,David H. Buss,Mark J. Pettenati +5 more
TL;DR: Ph+ AMLs are increasingly being reported with either M-BCR or m- BCR gene rearrangements, similar to those found with Ph+ ALL lending support to the notion that Ph+ AMls are distinct entities and not merely blastic phases of undiagnosed CML, further supported by the existence of Ph+ MDS cases.
Journal ArticleDOI
Analysis of protein tyrosine kinase expression in melanocytic lesions by tissue array.
TL;DR: It is proposed that protein tyrosine kinases (PTKs) may participate in this progression of melanoma from benign nevi to metastatic melanoma (MMM), and that this progression involves a multistep process.
Journal ArticleDOI
Improved sensitivity of BCR-ABL detection: a triple-probe three-color fluorescence in situ hybridization system.
TL;DR: The data show that the triple-probe/three-color approach allows highly sensitive detection of residual disease and this method is readily applicable to the analysis of other chromosome translocations.
Journal ArticleDOI
Variant Philadelphia translocations: Molecular-cytogenetic characterization and prognostic influence on frontline imatinib therapy, a GIMEMA working party on CML analysis
Giulia Marzocchi,Fausto Castagnetti,Simona Luatti,Carmen Baldazzi,Monica Stacchini,Gabriele Gugliotta,Marilina Amabile,Giorgina Specchia,Mario Sessarego,Ursula Giussani,Laura Valori,Giancarlo Discepoli,A. Montaldi,Alessandra Santoro,Laura Bonaldi,Giovanni Giudici,A. M. Cianciulli,Francesca Giacobbi,Francesca Palandri,Fabrizio Pane,Giuseppe Saglio,Giovanni Martinelli,Michele Baccarani,Gianantonio Rosti,Nicoletta Testoni +24 more
TL;DR: It is suggested that patients with variant translocations do not constitute a "warning" category in the imatinib era, regardless of the involvement of different mechanisms, the number of involved chromosomes, or the presence of deletions.
Journal ArticleDOI
Female phenotype and multiple abnormalities in sibs with a Y chromosome and partial X chromosome duplication: H--Y antigen and Xg blood group findings.
TL;DR: A mentally retarded female child with multiple congenital abnormalities had an abnormal X chromosome and a Y chromosome; the karyotype was interpreted as 46,dup(X)(p21 leads to pter)Y, a finding consistent with the view that testis-determining genes of the Y chromosome may be suppressed by regulatory elements of the X.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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