Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Fusion genes and their discovery using high throughput sequencing
TL;DR: The history of fusion genes in cancer and the ways in which fusion genes form and affect cellular function are described and computational methodologies for detecting fusion genes from high-throughput sequencing experiments are described.
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Synthesis, biological evaluation and docking studies of 4-amino substituted 1H-pyrazolo[3,4-d]pyrimidines.
Silvia Schenone,Chiara Brullo,Olga Bruno,Francesco Bondavalli,Luisa Mosti,Giovanni Maga,Emmanuele Crespan,Fabio Carraro,Fabrizio Manetti,Cristina Tintori,Maurizio Botta +10 more
TL;DR: Some compounds emerged as good dual inhibitors of the two enzymes, showed antiproliferative effects on two Bcr-Abl positive leukemia cell lines K-562 and KU-812, and induced apoptosis, as demonstrated by the PARP assay.
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Dual tyrosine kinase inhibitors in chronic myeloid leukemia.
TL;DR: The development, the mode of action and the preclinical or early clinical evaluation of several novel dual Src and Abl kinase inhibitors are reviewed, showing potential to eradicate residual disease and overcome or prevent resistance in chronic myeloid leukemia patients.
Journal ArticleDOI
Phosphotyrosine antibodies identify the p210c-abl tyrosine kinase and proteins phosphorylated on tyrosine in human chronic myelogenous leukemia cells.
Luigi Naldini,Alessandra Stacchini,Daniela Maria Cirillo,M. Aglietta,F Gavosto,Paolo M. Comoglio +5 more
TL;DR: Probed on human leukemia cell lines, phosphotyrosine antibodies recognized a 210,000-molecular-weight protein (p210) in K562 cells, a cell line derived from a Philadelphia (Ph)'-positive chronic myelogenous leukemia (CML), but recognized no protein in control Ph'-negative non-CML leukemia cells.
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Intergenically Spliced Chimeric RNAs in Cancer
Yuemeng Jia,Zhongqiu Xie,Hui Li +2 more
TL;DR: Current knowledge on chimeric RNAs and implications for cancer detection and treatment are reviewed, outstanding questions for the advancement of the field are discussed, and 'intergenically spliced' fusion RNAs are discussed.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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