Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Spectrum of somatic mutation dynamics in chronic myeloid leukemia following tyrosine kinase inhibitor therapy
TaeHyung Kim,Marc S. Tyndel,Hyeoung Joon Kim,Jae-Sook Ahn,Seunghyun Choi,Hee Jeong Park,Yeo-Kyeoung Kim,Soo Young Kim,Jeffrey H. Lipton,Zhaolei Zhang,Dennis Dong Hwan Kim +10 more
TL;DR: It is shown that patterns of mutation acquisition, persistence, and clearance vary but have a number of interesting correlations with clinical outcomes, and clinical implications of mutation profiles and dynamics in CML should be interpreted with caution.
Journal ArticleDOI
A non-Smad mechanism of fibroblast activation by transforming growth factor-β via c-Abl and Egr-1: selective modulation by imatinib mesylate
Swati Bhattacharyya,Wataru Ishida,Minghua Wu,Mark C. Wilkes,Yasuji Mori,Monique Hinchcliff,Edward B. Leof,John Varga +7 more
TL;DR: It is reported that when expressed in normal fibroblasts, a constitutively active mutant of Abl that causes chronic myelogenous leukemia (CML) stimulated the expression and transcriptional activity of the early growth response factor 1 (Egr-1) and was identified as a target of inhibition by imatinib.
Journal ArticleDOI
Extremely high and specific activity of DNA enzymes in cells with a Philadelphia chromosome
Masaki Warashina,Masaki Warashina,Tomoko Kuwabara,Tomoko Kuwabara,Yuka Nakamatsu,Yuka Nakamatsu,Kazunari Taira,Kazunari Taira +7 more
TL;DR: DNA enzymes with 2'-O-methyl modifications are potentially useful as gene-inactivating agents in the treatment of diseases such as CML.
Journal ArticleDOI
Expression of p210bcr/abl by metallothionein promoter induced T-cell leukemia in transgenic mice
Hiroaki Honda,Takeshi Fujii,Masaaki Takatoku,Hiroyuki Mano,Owen N. Witte,Yoshio Yazaki,Hisamaru Hirai +6 more
TL;DR: The results suggest that the tumorigenicity of the p210bcr/abl chimeric protein is restricted to the hematopoietic tissues in vivo and that an event enhancing p210a/abl expression contributed a proliferative advantage to hematoietIC precursor cells and eventually developed T-cell leukemia in transgenic mice.
Journal ArticleDOI
Chromosomes and causation of human cancer and leukemia. XXII. Karyotypic changes in malignant melanoma
TL;DR: Detailed karyotypic analysis with G‐ and C‐banding has been performed on cells of four malignant melanomas, finding Chromosome #1 was more frequently involved in aberrations than any other chromosome.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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