Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
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Journal ArticleDOI
Chromosomes and causation of human cancer and leukemia. XLV. Chromosome patterns in stimulated lymphocytes of chronic lymphocytic leukemia
TL;DR: EBV and LPS stimulate leukemic cells of CLL to undergo mitosis, about 50% of the patients have clonal abnormalities in the leukedmic cells, and CLL is associated with karyotypic changes seen in lymphoma (14q+), although the most common anomaly in the authors' material was a +12.
Journal ArticleDOI
CaMKII γ, a critical regulator of CML stem/progenitor cells, is a target of the natural product berbamine
Ying Gu,Ying Gu,Ting Chen,Zhipeng Meng,Yichao Gan,Xiaohua Xu,Guiyu Lou,Hongzhi Li,Xiaoxian Gan,Hong Zhou,Jinfen Tang,Gen-Bo Xu,Liansheng Huang,Xiaohong Zhang,Yongming Fang,Kai Wang,Shu Zheng,Wendong Huang,Rongzhen Xu,Rongzhen Xu +19 more
TL;DR: Berbamine is identified as a specific and critical target of berbamine for its antileukemia activity and may be the first ATP-competitive inhibitor of CaMKII γ, and potentially, can serve as a new type of molecular targeted agent through inhibition of the CaMK II γ activity for treatment of leukemia.
Book ChapterDOI
The Bcl-2 gene family and apoptosis
TL;DR: The evolutionarily conserved bcl-2 protein is recognized as being a member of a family of related proteins which can be categorized as death agonists or death antagonists, and the possible mechanisms of function which allow the bCl-2 family of proteins to either promote or suppress cell death.
Journal ArticleDOI
Structure-based optimization of pyrazolo[3,4-d]pyrimidines as Abl inhibitors and antiproliferative agents toward human leukemia cell lines.
Fabrizio Manetti,Chiara Brullo,M Magnani,Francesca Mosci,Beatrice Chelli,Emmanuele Crespan,Silvia Schenone,Antonella Naldini,Olga Bruno,Maria Letizia Trincavelli,Giovanni Maga,Fabio Carraro,Claudia Martini,Francesco Bondavalli,Maurizio Botta +14 more
TL;DR: Results from molecular docking calculations and Grid mapping laid the foundations for a structure-based optimization approach to improve the biological properties of pyrazolo-pyrimidine derivatives in terms of inhibition of Abl enzymatic activity and antiproliferative properties toward human leukemia cells.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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