Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Identification of Targetable FGFR Gene Fusions in Diverse Cancers
Yi-Mi Wu,Fengyun Su,Shanker Kalyana-Sundaram,Nickolay A. Khazanov,Bushra Ateeq,Xuhong Cao,Robert J. Lonigro,Pankaj Vats,Rui Wang,Su-Fang Lin,Ann Joy Cheng,Lakshmi P. Kunju,Javed Siddiqui,Scott A. Tomlins,Peter Wyngaard,Seth Sadis,Sameek Roychowdhury,Maha Hussain,Felix Y. Feng,Mark M. Zalupski,Moshe Talpaz,Kenneth J. Pienta,Daniel R. Rhodes,Dan R. Robinson,Arul M. Chinnaiyan +24 more
TL;DR: Because of the combinatorial possibilities of FGFR family fusion to a variety of oligomerization partners, clinical sequencing efforts are poised to identify rare, targetable FGFR fusions across diverse cancer types.
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The origin of human cancers.
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The biology of cml blast crisis
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TL;DR: Validation of the critical role of certain secondary changes (ie, loss of p53 or C/EBPalpha function) in murine models of CML blast crisis and in in vitro assays of BCR/ABL transformation of human hematopoietic progenitors might lead to the development of novel therapies based on targeting BCR /ABL and inhibiting or restoring the gene activity gained or lost during disease progression.
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The secret ally: immunostimulation by anticancer drugs
TL;DR: The molecular and cellular circuitries whereby cytotoxic agents can activate the immune system against cancer, and their therapeutic implications, are discussed.
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Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein
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TL;DR: It is established that P210bcr/abl can transform hematopoietic cell types to tumorigenicity and for autocrine production of interleukin 3 by factor-independent cell lines was found.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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