Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
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Patent
Method of detecting genetic translocations identified with chromosomal abnormalities
TL;DR: The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application as mentioned in this paper, and are used for in situ hybridization and stain both interphase and metaphase chromosomes with reliable signals.
Journal ArticleDOI
Requirement for antiapoptotic MCL-1 in the survival of BCR-ABL B-lineage acute lymphoblastic leukemia
Brian Koss,Jeffrey B. Morrison,Rhonda M. Perciavalle,Harpreet Singh,Harpreet Singh,Jerold E. Rehg,Richard T. Williams,Joseph T. Opferman +7 more
TL;DR: It is reported that endogenous MCL-1's antiapoptotic activity promotes survival during BCR-ABL transformation and in established BCR -ABL(+) leukemia cell lines.
Journal ArticleDOI
Detection of the molecular abnormality in chronic myeloid leukemia by use of the polymerase chain reaction.
TL;DR: The bcr-abl translocation characteristic of chronic myeloid leukemia was detected by the polymerase chain reaction (PCR) modified to use mRNA as the starting material, indicating the great sensitivity of the method.
Journal ArticleDOI
Frequent and extensive deletion during the 9,22 translocation in CML
TL;DR: The location of the deletions suggests that some mechanism maintains the chromosomal breakpoint on the Philadelphia chromosome within a limited region, and complicate the interpretation of Southern blots as a means of detecting the translocation.
Journal ArticleDOI
Low BCR-ABL expression levels in hematopoietic precursor cells enable persistence of chronic myeloid leukemia under imatinib
TL;DR: In this article, the number of BCR-ABL-positive precursors decreases significantly in all bone marrow compartments during major molecular remission (MMR), and they were able to demonstrate substantially lower BCRABL expression levels in persisting MMR colony-forming units (CFUs) compared with CML CFUs from diagnosis.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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