Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Lineage heterogeneity in acute leukaemia: acute mixed-lineage leukaemia and lineage switch.
Sanford Stass,Joseph Mirro +1 more
TL;DR: It is hypothesized that enzymes such as TdT, a DNA polymerase that catalyses polymerization of deoxyribonucleotides without a DNA template, could serve as a modifier of DNA sequences, permitting otherwise inactive genes to be expressed, and it is now clear that unusual combinations of myeloid and lymphoid cell lineages are much more common in acute leukaemia than have been generally recognized or suspected.
Journal ArticleDOI
The structure of the T cell antigen receptor genes in normal and malignant T cells.
TL;DR: In this review the genomic structure and the RNA transcripts of the alpha and beta chain of the T cell antigen receptor have been discussed and chromosome translocations involving the TcR alpha chain genes have been recognized.
Journal ArticleDOI
How does DNA break during chromosomal translocations
TL;DR: This review summarizes recent advances made in understanding the molecular mechanism of chromosomal translocations and considers lymphoma and leukemia as primary causes for cancers.
Journal ArticleDOI
Healthcare resource utilization and costs associated with non-adherence to imatinib treatment in chronic myeloid leukemia patients
Eric Q. Wu,Scott J. Johnson,Nicolas Beaulieu,Mateo Arana,Vamsi Bollu,Amy Guo,John Coombs,Weiwei Feng,Jorge E. Cortes +8 more
TL;DR: Better imatinib adherence was associated with significantly lower resource utilization and costs in CML patients, as lower imatinIB costs in low MPR patients were more than offset by higher non-imatinib costs mostly driven by inpatient services.
Journal ArticleDOI
BMS-214662 potently induces apoptosis of chronic myeloid leukemia stem and progenitor cells and synergizes with tyrosine kinase inhibitors.
Mhairi Copland,Francesca Pellicano,Linda Richmond,Elaine K. Allan,Ashley Hamilton,Francis Y. Lee,Roberto Weinmann,Tessa L. Holyoake +7 more
TL;DR: This is the first report of an agent with activity in resistant and blast crisis CML that selectively kills CML stem/progenitor cells through apoptosis and offers potential for eradication of chronic phase CML.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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