Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Dasatinib (BMS-354825) targets an earlier progenitor population than imatinib in primary CML but does not eliminate the quiescent fraction
Mhairi Copland,Ashley Hamilton,Lucy J. Elrick,Janet W. Baird,Elaine K. Allan,Niove E. Jordanides,Martin Barow,Joanne C. Mountford,Tessa L. Holyoake +8 more
TL;DR: It is confirmed that dasatinib is more effective than IM within the CML stem cell compartment; however, the most primitive quiescent CML cells appear to be inherently resistant to both drugs.
PatentDOI
Recurrent gene fusions in prostate cancer
Scott A. Tomlins,Daniel R. Rhodes,Arul M. Chinnaiyan,Rohit Mehra,Mark A. Rubin,Xiao-Wei Sun,Sven Perner,Charles M.C. Lee,Francesca Demichelis +8 more
TL;DR: Recurrent gene fusions of androgen regulated genes and ETS family member genes in prostate cancer are described in this paper, where compositions and methods having utility in prostate diagnosis, research, and therapy are also provided.
Journal ArticleDOI
Translation of the Philadelphia chromosome into therapy for CML.
TL;DR: The understanding of resistance to imatinib has rapidly led to strategies to circumvent resistance, which will allow this paradigm of targeting molecular pathogenetic events to be applied to many additional hematologic cancers.
Journal ArticleDOI
The colorectal adenoma–carcinoma sequence
TL;DR: The evidence supporting the hypothesis that the adenoma–carcinoma sequence represents the process by which most, if not all, colorectal cancers arise has increased rapidly in recent years and is reviewed critically.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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