Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
TopHat-Fusion: an algorithm for discovery of novel fusion transcripts.
TL;DR: TopHat-Fusion is an enhanced version of TopHat, an efficient program that aligns RNA-seq reads without relying on existing annotation and can discover fusion products deriving from known genes, unknown genes and unannotated splice variants of known genes.
Journal ArticleDOI
PAX8-PPARγ1 Fusion in Oncogene Human Thyroid Carcinoma
Todd G. Kroll,Pasha Sarraf,Lorenza Pecciarini,Chang-Jie Chen,Elisabetta Mueller,Bruce M. Spiegelman,Jonathan A. Fletcher,Jonathan A. Fletcher,Jonathan A. Fletcher +8 more
TL;DR: T(2;3)(q13;p25), a translocation identified in a subset of human thyroid follicular carcinomas, results in fusion of the DNA binding domains of the thyroid transcription factor PAX8 to domains A to F of the peroxisome proliferator–activated receptor (PPAR) γ1.
Journal ArticleDOI
Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro.
Ernest S. Kawasaki,Steven S. Clark,Mazie Y. Coyne,Stephen D. Smith,Richard E. Champlin,Owen N. Witte,Frank McCormick +6 more
TL;DR: D diagnosis of chronic myeloid and acute lymphocytic leukemias by this procedure is rapid, much more sensitive than existing protocols, and independent of the presence or absence of an identifiable Philadelphia chromosome.
Journal ArticleDOI
Translocation of c- abl oncogene correlates with the presence of a Philadelphia chromosome in chronic myelocytic leukaemia
Claus R. Bartram,Annelies de Klein,Anne Hagemeijer,Ton van Agthoven,Ad Geurts van Kessel,Dirk Bootsma,Gerard Grosveld,Malcolm A. Ferguson-Smith,Teresa Davies,Marion Stone,Nora Heisterkamp,John R. Stephenson,John Groffen +12 more
TL;DR: In this article, the translocation of the human cellular homologue (c-ab1) of the transforming sequence of Abelson murine leukaemia virus (A-MuLV) from chromosome 9 to the Philadelphia chromosome (Ph1) in chronic myelocytic leukemiaemia (CML) was investigated.
Journal ArticleDOI
Acute leukaemia in bcr/abl transgenic mice
TL;DR: Mice transgenic for a bcr/abl p190 DNA construct are generated and it is found that progeny are either moribund with, or die of acute leukaemia (myeloid or lymphoid) 1058 days after birth, evidence for a causal relationship between the Philadelphia chromosome and humanLeukaemia.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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