Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Granulocyte-Macrophage Progenitors as Candidate Leukemic Stem Cells in Blast-Crisis CML
Catriona Jamieson,Laurie Ailles,Scott J. Dylla,Manja Muijtjens,Carol D. Jones,James L. Zehnder,Jason Gotlib,Kevin Li,Markus G. Manz,Armand Keating,Charles L. Sawyers,Irving L. Weissman +11 more
TL;DR: Activation of beta-catenin in CML granulocyte-macrophage progenitors appears to enhance the self-renewal activity and leukemic potential of these cells.
Journal ArticleDOI
A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia
Annelies de Klein,Ad Geurts van Kessel,Gerard Grosveld,Claus R. Bartram,Anne Hagemeijer,Dirk Bootsma,Nigel K. Spurr,Nora Heisterkamp,John Groffen,John R. Stephenson +9 more
TL;DR: Positive hybridization is found when the 22q−(the Philadelphia chromosome), and not the 9q+ derivative of the translocation, is present in the cell hybrids, and this finding is a direct demonstration of a reciprocal exchange between the two chromosomes and suggests a role for the c-abl gene in the generation of CML.
Journal ArticleDOI
Glivec (STI571, imatinib), a rationally developed, targeted anticancer drug
TL;DR: This work describes how this drug discovery programme led to the discovery and continuing development of Glivec (Gleevec in the United States), the first selective tyrosine-kinase inhibitor to be approved for the treatment of a cancer.
Journal ArticleDOI
The impact of translocations and gene fusions on cancer causation
TL;DR: An analysis of available data shows that gene fusions occur in all malignancies, and that they account for 20% of human cancer morbidity, with the advent of new and powerful investigative tools that enable the detection of cytogenetically cryptic rearrangements.
Journal ArticleDOI
Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation
TL;DR: The results suggest that t(14;18) translocations alter expression of the bcl-2 gene both by transcriptional activation and by abnormal posttranscriptional regulation of bCl-2 mRNA.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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