Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Cytological and cytogenetical studies on brain tumors. 4. Identification of the missing G chromosome in human meningiomas as no. 22 by fluorescence technique.
H. Zankl,K. D. Zang +1 more
TL;DR: Among 70 human meningiomas cytogenetically investigated by us up till now, only 4 tumors showed a hyperdiploidy, and 2 of them had a uniform stemline with 47 chromosomes (47,XX,G+ and 47, XY, C(?E)+); the other 2 menediomas had a modal number of 53 (55) chromosomes.
Journal ArticleDOI
Advances in understanding and management of myeloproliferative neoplasms.
TL;DR: A clinically oriented overview of myeloproliferative neoplasms in terms of their molecular pathogenesis, classification, diagnosis, and management is provided in this article. But the authors do not provide a classification of MPNs.
Journal ArticleDOI
Clonal haematopoiesis: connecting ageing and inflammation in cardiovascular disease
Siddhartha Jaiswal,Peter Libby +1 more
TL;DR: It is proposed that the mutations leading to clonal haematopoiesis contribute to the increased inflammation seen in ageing and thereby explain some of the age-related risk of cardiovascular disease.
Journal ArticleDOI
BCR/ABL induces multiple abnormalities of cytoskeletal function.
Ravi Salgia,Jian-Liang Li,Darren S. Ewaniuk,Warren S. Pear,Evan Pisick,S. A. Burky,T J Ernst,Martin Sattler,Lan Bo Chen,James D. Griffin +9 more
TL;DR: Time-lapse video microscopy was used to study cell motility and cell morphology on extracellular cell matrix protein-coated surfaces of a series of cell lines before and after transformation by BCR/ABL, finding a striking increase in spontaneous Motility and other defects of cytoskeletal function.
Journal ArticleDOI
Chromosome Translocation, B Cell Lymphoma, and Activation-Induced Cytidine Deaminase
TL;DR: The discovery of activation-induced cytidine deaminase (AID) and the use of murine models to study translocation have led to a new understanding of how these events contribute to the genesis of lymphomas.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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