Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Comparable complex rearrangements involving 8;21 and 9;22 translocations in leukaemia.
Valerie Lindgren,Janet D. Rowley +1 more
TL;DR: It is reported that, when more complex structural changes occur, com parable patterns of rearrangements are found for both the 9;22 and the 8;21 translocations, which provides further sup port for Boveri's hypothesis that chromosomal alterations are among the fundamental changes that result in malignancy.
Journal ArticleDOI
Immunohistochemical Analysis of Platelet-derived Growth Factor Receptor-α, -β, c-kit, c-abl, and Arg Proteins in Glioblastoma: Possible Implications for Patient Selection for Imatinib Mesylate Therapy
Christine Haberler,Ellen Gelpi,Christine Marosi,Karl Rössler,Peter Birner,Herbert Budka,J. A. Hainfellner,J. A. Hainfellner +7 more
TL;DR: It is shown here for the first time in a large series of glioblastomas that PDGFR-α, -β, c-kit,c-abl and arg expression is immunohistochemically detectable in a fraction of cases.
Journal ArticleDOI
hTERT, the catalytic component of telomerase, is downregulated in the haematopoietic stem cells of patients with chronic myeloid leukaemia.
Lisa J. Campbell,Carrie Fidler,Helen Eagleton,A J Peniket,Rajko Kusec,Shira Gal,Timothy Littlewood,J. S. Wainscoat,Jacqueline Boultwood +8 more
TL;DR: It is suggested that reduced hTERT expression directly causes the shortened telomeres observed in CML.
Journal ArticleDOI
Lymphocyte surface immunoglobulins in Hodgkin's disease.
Journal ArticleDOI
Lipid nanoparticle-mediated siRNA delivery for safe targeting of human CML in vivo.
Nidhi Jyotsana,Amit Sharma,Anuhar Chaturvedi,Ramachandramouli Budida,Michaela Scherr,Florian Kuchenbauer,Robert Lindner,Fatih Noyan,Kurt-Wolfram Sühs,Martin Stangel,Denis Grote-Koska,Korbinian Brand,Hans-Peter Vornlocher,Matthias Eder,Felicitas Thol,Arnold Ganser,R. Keith Humphries,R. Keith Humphries,Euan Ramsay,Pieter R. Cullis,Michael Heuser +20 more
TL;DR: This study developed DLin-MC3-DMA lipid-based LNP-siRNA formulations for systemic delivery against a driver oncogene to target human chronic myeloid leukemia (CML) cells in vivo and shows a highly efficient and non-toxic delivery of siRNA in vitro and in vivo.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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